首页> 外文期刊>DTW:Deutsche Tierarztliche Wochenschrift >Immunohistological demonstration of Rhodococcus equi in a trotter foal.
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Immunohistological demonstration of Rhodococcus equi in a trotter foal.

机译:猪蹄马中红红球菌的免疫组织学证明。

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摘要

A 3-month-old female trotter foal was euthanized due to severe dyspnoea. Pathomorphologically a chronic granulomatous to necrotizing pneumonia was found and Rhodoccocus (R.) equi was isolated microbiologically. An immunohistological method using a murine monoclonal antibody against a 15-17 kDa antigen of virulent R. equi was established in formalin-fixed and paraffin-embedded tissue sections using various antigen retrieval techniques to optimize the staining results. Microwave treatment was most suitable for the demonstration of bacterial antigen localized predominantly in intralesional macrophages. Immunohistology is an additional method for identifying R. equi-infections in equine tissue and may be useful in retrospective studies on paraffin-embedded archive material.
机译:由于严重的呼吸困难,对3个月大的母猪小马驹实施了安乐死。在病理形态学上,发现了一种慢性肉芽肿至坏死性肺炎,并通过微生物学方法分离了红球菌(Rhodoccocus)(i.R。) equi 。一种免疫组学方法,使用针对有毒iR的15-17 kDa抗原的鼠单克隆抗体。在福尔马林固定和石蜡包埋的组织切片中使用各种抗原修复技术建立了equip,以优化染色结果。微波处理最适合于证明细菌抗原主要位于病灶内巨噬细胞中。免疫组织学是鉴定R的另一种方法。马组织中的马感染,可能有助于对石蜡包埋的档案材料进行回顾性研究。

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