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首页> 外文期刊>DTW:Deutsche Tierarztliche Wochenschrift >Clostridium perfringens toxin types from freshwater fishes in one water reservoir of Shandong Province of China, determined by PCR.
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Clostridium perfringens toxin types from freshwater fishes in one water reservoir of Shandong Province of China, determined by PCR.

机译：PCR检测山东省一个水库淡水鱼的产气荚膜梭菌毒素类型。

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摘要

This study was conducted to determine the occurrence and prevalence of Clostridium perfringens toxin types and toxins in some freshwater fish like carps, silver carps, crucian carps, catfishes and zaieuws caught from Shengli Water Reservoir, Tainan, Shandong, China. 420 intestinal content samples (not including intestinal tissues) of freshwater fishes (60 silver carps, 100 carps, 100 crucian carps, 60 catfishes and 100 zaieuws) were examined bacteriologically for the occurrence of C. perfringens using PCR for genes encoding the four lethal toxins ( alpha , beta , epsilon and iota ) for toxin classification and for genes encoding enterotoxin and the novel beta 2 toxin for further subclassification. C. perfringens was isolated from 75 intestinal content samples (17.9%) from 13 silver carps, 2 carps, 12 crucian carps, 40 zaieuws, and 8 catfishes. Out of the 75 isolates, 58 strains (77.3%) were C. perfringens toxin type C ( alpha and beta toxin positive), 13 strains (17.3%) were toxin type A ( alpha toxin positive) and 4 strains (5.3%) were toxin type B ( alpha , beta and epsilon toxin positive). In addition, the gene encoding for beta 2 toxin was found in 47 strains (62.7%) of all the isolates, seven from type A, two from type B, and 38 from type C. The gene encoding for enterotoxin was not found in any isolate. These amplified toxin gene fragments which were cloned, sequenced and compared with reference strains, revealed that the identity varied from 98.15-99.29%. This is the first report of C. perfringens alpha , beta , epsilon , beta 2 toxins in freshwater fish and of beta , epsilon toxins in fish in general, and is the first discovery that the beta 2 toxin can be detected in type B strains. The origin of this bacterium and its importance to human food poisoning in freshwater fish is discussed.
机译：这项研究旨在确定产自山东台南胜利水库的淡水鱼如鲤鱼，银鲤，cru鱼，cat鱼和zaieuws中的产气荚膜梭菌毒素类型和毒素的发生和流行情况。中国。对420只淡水鱼（60条银鲤，100条鲤鱼，100条cru鱼，60条fish鱼和100条zaieuws）的肠内容物样品（不包括肠组织）进行了细菌学检查，发现是否存在C。使用PCR对编码四种致命毒素（α，β，ε和iota）的基因进行毒素分类，并对编码肠毒素和新型β2毒素的基因进行进一步亚分类。 C。从13个银鲤，2个鲤鱼，12个cru鱼，40个zaieuws和8个cat鱼的75个肠内容物样品中分离了产气荚膜杆菌。在75个分离株中，58个菌株（77.3％）是C。产气荚膜毒素C型（α和β毒素阳性），13株（17.3％）是A型毒素（α毒素阳性）和4株（5.3％）是B型毒素（α，β和ε毒素阳性） ）。此外，在所有分离株的47个菌株（62.7％）中发现了编码β2毒素的基因，其中7个来自A型，两个来自B型，以及38个来自C型。在任何菌株中均未发现编码肠毒素的基因。隔离。这些扩增的毒素基因片段被克隆，测序并与参考菌株进行比较，揭示出同一性在98.15-99.29％之间。这是 C的第一份报告。淡水鱼中的perfringensα，β，ε，β2毒素以及一般鱼类中的β，ε毒素，这是第一个发现可以在B型菌株中检测到β2毒素的发现。讨论了这种细菌的起源及其对淡水鱼中人类食物中毒的重要性。

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