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首页> 外文期刊>DTW:Deutsche Tierarztliche Wochenschrift >Diagnosis of lawsonia intracellularis infection in pigs after vaccination or antimicrobial treatment
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Diagnosis of lawsonia intracellularis infection in pigs after vaccination or antimicrobial treatment

机译：疫苗接种或抗菌治疗后猪胞内劳顿菌感染的诊断

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Diagnosis of infection with Lawsonia (L.) intracellularis, the causative agent of porcine proliferative enteropathy, is routinely based on the detection of L. intracellularis in faeces and/or tissue samples by PCR. Furthermore, infection can be determined by the detection of antibodies in serum samples. The aim of this comparative study was to examine whether results from molecular biology and serological testing are influenced by applying live bacteria vaccine Enterisol Ileitis or antimicrobial treatment to pigs naturally exposed to L. intracellularis. Decreased shedding was detected in tylosin treated pigs only. Thus, the distribution of 345 pigs into groups of 0-, 1- or > or = 2-times positive by PCR was significantly influenced by the treatment (P < 0.0001). A global effect of positive PCR results on average daily weight gain was also highly significant (P < 0.0001). The serological reaction was detected by a blocking ELISA after infection but not after vaccination independent of treatment. The results indicate no influence of vaccination on applied diagnostic tests.
机译：猪增生性肠病的病原体细胞内劳森氏菌感染的诊断通常基于通过PCR检测粪便和/或组织样本中的细胞内劳氏菌。此外，可以通过检测血清样品中的抗体来确定感染。这项比较研究的目的是研究对自然暴露于细胞内劳森氏菌的猪进行活菌疫苗肠溶性回肠炎或抗菌治疗是否会影响分子生物学和血清学测试的结果。仅在经泰乐菌素处理的猪中检测到脱落减少。因此，治疗显着影响了345头猪被PCR呈0、1，或>或= 2倍阳性的分布（P <0.0001）。 PCR阳性结果对日均增重的总体影响也非常显着（P <0.0001）。感染后通过阻断ELISA检测血清学反应，但接种后与治疗无关而未检测到。结果表明疫苗接种对应用的诊断测试没有影响。

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