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首页> 外文期刊>DTW:Deutsche Tierarztliche Wochenschrift >Clostridium perfringens toxin types from freshwater fishes in one water reservoir of Shandong Province of China, determined by PCR.
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Clostridium perfringens toxin types from freshwater fishes in one water reservoir of Shandong Province of China, determined by PCR.

机译：PCR检测山东省一个水库淡水鱼类产气荚膜梭菌毒素的类型。

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Four hundred and twenty intestinal content samples (not including intestinal tissues) of freshwater fishes (60 silver carps, 100 carps, 100 crucian carps, 60 catfishes and 100 zaieuws) caught from one water reservoir were examined bacteriologically for the occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (alpha, beta, epsilon and iota) for classification into toxin types and for genes encoding enterotoxin and the novel beta2 toxin for further subclassification. C. perfringens could be isolated in 75 intestinal contents samples (17.9%) from freshwater fish including: 13 silver carps, 2 carps, 12 crucian carps, 40 zaieuws, and 8 catfishes. In 75 isolates, 58 strains (77.3%) were C. perfringens toxin type C (alpha and beta toxin positive), 13 strains (17.3%) were toxin type A (alpha toxin positive) and 4 strains (5.3%) were toxin type B (alpha, beta and epsilon toxin positive). In addition, the gene encoding for beta2 toxin was found in 47 strains (62.7%) of all the isolates, seven from type A, two from type B, and 38 from type C. The gene encoding for enterotoxin was not found in any isolate. These amplified toxin gene fragment were cloned and sequenced and compared with reference strains, the identity varied from 98.15% to 99.29%. This is the first report of C. perfringens alpha, beta, epsilon, beta2 toxins in freshwater fish and of beta, epsilon toxins in fish in general, and is the first discovery that the beta2 toxin could be detected in strains of type B. The origin of this bacterium and its importance to human food poisoning in freshwater fish is discussed.
机译：从细菌学上检查了从一个水库中捕获的淡水鱼（60条银鲤，100条鲤鱼，100条cru鱼，60条cat鱼和100条鱼）的肠含量样本（不包括肠组织），进行了细菌学检查，以确定产气荚膜梭菌的发生。通过聚合酶链反应（PCR）检查分离物的编码四种致命毒素（α，β，ε和iota）的基因以将其分类为毒素类型，并将编码肠毒素的基因和新的beta2毒素进行进一步的亚分类。产气荚膜梭菌可以从淡水鱼中分离出75个肠内容物样本（占17.9％），包括：13条银鲤，2条鲤，12条cru鱼，40条zaieuws和8条ws鱼。在75株中，58株（77.3％）是产气荚膜梭菌毒素C型（α和β毒素阳性），13株（17.3％）是A型毒素（α毒素阳性），4株（5.3％）是毒素型B（α，β和ε毒素阳性）。此外，在所有分离株的47株（62.7％）中发现了编码β2毒素的基因，其中7株来自A型，2株来自B型，38株来自C型。在任何分离株中均未发现编码肠毒素的基因。 。将这些扩增的毒素基因片段克隆并测序，并与参考菌株进行比较，其同一性从98.15％到99.29％不等。这是淡水鱼中的产气荚膜梭菌α，β，ε，β2毒素以及一般鱼类中β，ε毒素的首次报道，也是首次发现可以在B型菌株中检测到β2毒素的报道。讨论了这种细菌的起源及其对淡水鱼食物中毒的重要性。

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