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首页> 外文期刊>JAIDS: Journal of acquired immune deficiency syndromes >Active simian immunodeficiency virus (strain smmPGm) infection in macaque central nervous system correlates with neurologic disease.
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Active simian immunodeficiency virus (strain smmPGm) infection in macaque central nervous system correlates with neurologic disease.

机译：猕猴中枢神经系统中的主动猿猴免疫缺陷病毒（smmPGm株）感染与神经系统疾病有关。

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摘要

Simian immunodeficiency virus strain smmPGm can induce neuropathology in macaques and is a model for the development of human HIV-related brain injury. For quantitative studies of proviral presence and expression in the central nervous system (CNS), we inoculated 8 macaques intravenously with the virus. Three animals were necropsied 2 to 4 weeks after development of infection, and we obtained lymphoid tissue biopsies from 5 animals before 5 weeks after infection. Peak plasma viral loads averaged 10 viral RNA Eq/mL at week 2, whereas cerebrospinal fluid viral loads peaked at 10 viral RNA Eq/mL. The proviral DNA loads and viral gag mRNA expression in tissues were quantified by real-time polymerase chain reaction. Two animals developed neurologic disease characterized by meningoencephalitis and meningitis. Proviral DNA levels in CNS tissues of these animals at necropsy revealed 10 and 10 copies/microg of DNA, respectively, whereas viral RNA expression in the CNS reached 100 to 1000 times higher levels than those seen in early necropsies. In sharp contrast, in 2 animals necropsied at later times without CNS disease, virus mRNA expression was not detected in any CNS tissue. Our results are consistent with the hypothesis that active virus expression in the CNS is strongly correlated with neurologic disease and that the event occurs at variable periods after infection.
机译：猿猴免疫缺陷病毒株smmPGm可以诱发猕猴的神经病理，是人类HIV相关脑损伤发展的模型。为了定量研究中枢神经系统（CNS）中前病毒的存在和表达，我们用病毒静脉注射了8只猕猴。在感染发生后2至4周对3只动物进行尸检，在感染5周前从5只动物中获得淋巴组织活检。在第2周，血浆血浆病毒负荷峰值平均为10病毒RNA Eq / mL，而脑脊液病毒负荷峰值为10病毒RNA Eq / mL。通过实时聚合酶链反应对组织中的前病毒DNA负荷和病毒gag mRNA表达进行定量。两只动物发展出以脑膜脑炎和脑膜炎为特征的神经系统疾病。尸检时，这些动物的CNS组织中的原病毒DNA水平分别显示10和10拷贝/微克DNA，而CNS中病毒RNA的表达水平比早期尸检中的高100到1000倍。与之形成鲜明对比的是，在后来没有进行CNS疾病尸检的2只动物中，在任何CNS组织中均未检测到病毒mRNA表达。我们的结果与以下假设相符：在CNS中活跃的病毒表达与神经系统疾病密切相关，并且该事件在感染后的不同时期发生。

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