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首页> 外文期刊>JALA: Journal of the Association for Laboratory Automation >Optimization of the Separation of NDA-Derivatized Methylarginines by Capillary and Microchip Electrophoresis
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Optimization of the Separation of NDA-Derivatized Methylarginines by Capillary and Microchip Electrophoresis

机译：毛细管电泳和微芯片电泳分离NDA衍生的甲基精氨酸的分离优化

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The methylated arginines (MAs) monomethylarginine (MMA), asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA) have been shown to be independent predictors of cardiovascular disease. This article describes progress regarding the development of an analytical method capable of rapidly analyzing MAs using capillary electrophoresis (CE) and microchip electrophoresis (MCE) with laser-induced fluorescence (LIF) detection. Several parameters including buffer composition and separation voltage were optimized to achieve an ideal separation. The analytes of interest were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) to produce fluorescent 1-cyanobenz[f]isoindole (CBI) derivatives and then subjected to CE analysis. Baseline resolution of SDMA, ADMA, MMA, and arginine was achieved in less than 8 min. The limits of detection for SDMA, ADMA, MMA, and arginine were determined to be 15, 20, 25, and 5 nM, respectively, which are well below the expected plasma concentrations. The CE separation method was then transferred to a glass MCE device with LIF detection. MAs were baseline resolved in 3 min on-chip using a 14 cm separation channel with detection limits of approximately 10 nM for each species. To the best of the authors' knowledge, this is the first report of the separation of MAs by MCE.
机译：甲基化的精氨酸（MAs）单甲基精氨酸（MMA），不对称的二甲基精氨酸（ADMA）和对称的二甲基精氨酸（SDMA）已被证明是心血管疾病的独立预测因子。本文介绍了有关能够使用毛细管电泳（CE）和微芯片电泳（MCE）以及激光诱导荧光（LIF）检测快速分析MA的分析方法的进展。优化了包括缓冲液组成和分离电压在内的几个参数，以实现理想的分离效果。用萘-2,3-二甲醛（NDA）衍生化目标分析物，以生成荧光的1-氰基苯并[f]异吲哚（CBI）衍生物，然后进行CE分析。在不到8分钟的时间内即可达到SDMA，ADMA，MMA和精氨酸的基线分辨率。 SDMA，ADMA，MMA和精氨酸的检出限分别确定为15、20、25和5 nM，远低于预期的血浆浓度。然后将CE分离方法转移到具有LIF检测功能的玻璃MCE设备中。使用14 cm分离通道在芯片上3分钟内对MA进行基线分离，每个物种的检测限约为10 nM。就作者所知，这是MCE分离MA的第一份报告。

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