...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Herzogia >DNA barcodes for the distinction of reindeer lichens: a case study using Cladonia rangiferina and C. stygia
【24h】

DNA barcodes for the distinction of reindeer lichens: a case study using Cladonia rangiferina and C. stygia

机译：DNA条形码用于区分驯鹿地衣：使用Cladonia rangiferina和C. stygia的案例研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Many groups of lichens are difficult to identify due to their phenotypical plasticity. Molecular methods are therefore increasingly used not only to elucidate phylogenetic relationships among taxa, but also to assign individuals to species. Most members of the genus Cladonia are conspicuous and frequently collected, but notoriously variable. At the same time the genus comprises species that have to be regularly monitored under EU legislation. A "DNA barcoding" approach could thus be very helpful to correctly identify taxa. The internal transcribed spacer region of the ribosomal RNA repeat has been proposed as a universal barcoding marker for fungi. However, it has also been shown that data from this locus alone might be insufficient to reliably distinguish members of Cladonia. Taking the two similar species C. rangiferina and C. stygia as an example, we tested the suitability of ITS and five additional gene loci to distinguish species of Cladonia subgenus Cladina. Infraspecific variability was high in most of the investigated loci. Bayesian species delimitation using a multispecies coalescent approach based on multilocus DNA sequence data allowed us to separate the two species with high statistical support. The clustering of samples was also supported by phenotypic differences between the groups and a phylogenetic analysis. This allowed us to test the usefulness of single gene loci as barcoding markers on an objective basis. The only locus, for which minimum interspecific divergence exceeded maximum infraspecific variability in both species, was the small subunit of the mitochondrial ribosomal RNA repeat. Whether this locus should be suggested as an alternative or additional barcoding marker for the separation of Cladonia species needs to be tested with a broader taxonomic sample.
机译：许多类地衣由于表型可塑性而难以识别。因此，越来越多地使用分子方法来阐明分类群之间的系统发生关系，而且将个体分配给物种。 Cladonia属的大多数成员都是引人注目的且经常收集，但众所周知地是可变的。同时，该属包括必须根据欧盟法律进行定期监视的物种。因此，“ DNA条形码”方法对于正确识别分类单元可能非常有帮助。已经提出了核糖体RNA重复序列的内部转录间隔区作为真菌的通用条形码标记。但是，也已经表明，仅来自该基因座的数据可能不足以可靠地区分Cladonia成员。以两个相似的C. rangiferina和C. stygia物种为例，我们测试了ITS和五个其他基因位点以区分Cladonia亚属Cladina物种的适用性。在大多数调查的基因座中，种下变异性很高。使用基于多基因座DNA序列数据的多物种合并方法进行贝叶斯物种划分，使我们能够在高度统计支持下分离这两个物种。各组之间的表型差异和系统发育分析也支持了样品的聚类。这使我们能够客观地测试单个基因位点作为条形码标记的有效性。在两个物种中，最小种间差异超过最大种下变异性的唯一基因座是线粒体核糖体RNA重复序列的小亚基。是否应建议将此基因位点作为分离枝lad的替代或附加条形码标记，需要使用更广泛的分类学样本进行测试。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号