• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Japanese Journal of Ophthalmology >Expression of rinx/vsx1 during postnatal eye development in cone-bipolar, differentiating ganglion, and lens fiber cells.
【24h】

Expression of rinx/vsx1 during postnatal eye development in cone-bipolar, differentiating ganglion, and lens fiber cells.

机译：圆锥双极，分化神经节和晶状体纤维细胞在产后眼睛发育过程中rinx / vsx1的表达。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

PURPOSE: To investigate the expression pattern of the homeobox transcription factor Rinx (also referred to as Vsx1) during postnatal eye development of the mouse. METHODS: We cloned the mouse Rinx gene, inferred the sequence of the encoded protein, and prepared polyclonal antibodies against it. Immunohistochemical analysis (IHC) and in situ hybridization were employed to localize Rinx in postnatal and adult mouse eyes. Double-labeled IHC either with anti-protein kinase C (PKC, a marker of rod bipolar cells) or anti-vimentin (a marker of Muller glial cells) antibodies was performed in the adult retina. Rinx mRNA was also analyzed by reverse transcription-polymerase chain reaction in the lens. RESULTS: At P0 and P4 (postnatal days), Rinx-immunoreactive cells included retinal ganglion cells (RGC), cells of the innermost nuclear layer (INL) and of presumptive INL, differentiating lens fiber cells, and a few cells of the presumptive outer nuclear layer (ONL). At P8, both Rinx protein and mRNA were detected in the middle of the INL, and in RGC, but not in the lens. When the mice were 8 weeks of age, only a subset of nuclei in the outer half of the INL expressed both Rinx protein and mRNA. Double-labeling IHC indicated that Rinx- and either PKC- or vimentin-labeled cells were not colocalized. Therefore, Rinx is most likely expressed in adult cone bipolar cells and possibly in horizontal cells. CONCLUSIONS: Rinx may play important roles in the differentiation and maintenance of cone bipolar cells. Rinx is unique among members of this family of homeodomain proteins in that it may also be involved in differentiation of RGC and lens fiber cells.
机译：目的：探讨同源盒转录因子Rinx（也称为Vsx1）在小鼠产后眼睛发育过程中的表达模式。方法：我们克隆了小鼠Rinx基因，推断了编码蛋白的序列，并制备了针对它的多克隆抗体。免疫组织化学分析（IHC）和原位杂交被用于在产后和成年小鼠眼中定位Rinx。在成年视网膜中用抗蛋白激酶C（杆状双极细胞标记）或抗波形蛋白（穆勒胶质细胞标记）双标记IHC抗体。还通过晶状体中的逆转录-聚合酶链反应分析了Rinx mRNA。结果：在P0和P4（产后天），Rinx免疫反应性细胞包括视网膜神经节细胞（RGC），最内核层（INL）和推测性INL的细胞，分化的晶状体纤维细胞以及推测的外层细胞核层（ONL）。在P8处，在INL中部和RGC中均检测到Rinx蛋白和mRNA，但在晶状体中未检测到。当小鼠8周大时，仅INL外半部分的核子集同时表达Rinx蛋白和mRNA。双重标记的IHC指示Rinx和PKC或波形蛋白标记的细胞未共定位。因此，Rinx最有可能在成年锥体双极细胞中表达，也可能在水平细胞中表达。结论：Rinx可能在锥体双极细胞的分化和维持中起重要作用。 Rinx在该同源域蛋白家族中是独特的，因为它也可能参与RGC和晶状体纤维细胞的分化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号