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首页> 外文期刊>Journal of Analytical Toxicology >Methylation of 3,4-methylenedioxymethamphetamine in formalin-fixed human liver tissue.
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Methylation of 3,4-methylenedioxymethamphetamine in formalin-fixed human liver tissue.

机译：福尔马林固定的人肝组织中3,4-亚甲二氧基甲基苯丙胺的甲基化。

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摘要

3,4-Methylenedioxymethamphetamine (MDMA or ecstasy) is a commonly consumed recreational drug. As is the case with most secondary amines, MDMA reacts with formaldehyde under acidic conditions to form tertiary amines. This reaction is likely to occur in formalin-fixed tissue. In formalin solutions, MDMA is methylated producing 3,4-methylenedioxy-N,N-dimethylamphetamine (MDDA). MDDA standard was synthesized by treating methylenedioxyamphetamine HCl in formaldehyde solution. Structure confirmation was by electrospray ionization-mass spectrometry (MS) and MS-MS. Randomly chosen human liver pieces (100-200 mg) were injected with 2 microg of MDMA HCl. The liver pieces in centrifuge tubes were covered with 200 microL of formalin solution (20% v/v), held at room temperature for 24 h, and then homogenized. The resulting suspension was sonicated for 5 min and then centrifuged. Controls consisted of substitution of 200 microL of water in place of formalin solution. Supernatant aliquots (10 mciroL) were added to 500 microL of 0.1% formic acid in acetonitrile for MS analysis. Positive ion electrospray spectra recorded in MS, MS2, and MS3 modes were used to confirm the presence of methylated MDMA. Liver tissue containing added MDMA HCl but not treated with formalin did not show a detectable level of methylated MDMA.
机译：3，4-亚甲基二氧基甲基苯丙胺（MDMA或摇头丸）是一种常用的休闲药物。与大多数仲胺一样，MDMA在酸性条件下与甲醛反应形成叔胺。该反应很可能发生在福尔马林固定的组织中。在福尔马林溶液中，MDMA被甲基化，生成3,4-亚甲二氧基-N，N-二甲基苯丙胺（MDDA）。通过在甲醛溶液中处理亚甲基二氧基苯丙胺盐酸盐来合成MDDA标准品​​。通过电喷雾电离质谱（MS）和MS-MS确认结构。随机选择的人肝碎片（100-200 mg）注射2微克的MDMA HCl。离心管中的肝脏碎片用200微升福尔马林溶液（20％v / v）覆盖，在室温下放置24小时，然后匀浆。将得到的悬浮液超声处理5分钟，然后离心。对照包括代替福尔马林溶液的200微升水。将上清液等分试样（10 mciroL）添加到500 µL乙腈中的0.1％甲酸中，用于MS分析。以MS，MS2和MS3模式记录的阳离子电喷雾光谱用于确认甲基化MDMA的存在。含有添加的MDMA HCl但未用福尔马林处理的肝组织未显示可检测水平的甲基化MDMA。

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