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首页> 外文期刊>Journal of Analytical Toxicology >The detection of Delta9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-Delta9-tetrahydrocannabinol (THCA) in whole blood using two-dimensional gas chromatography and EI-mass spectrometry.
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The detection of Delta9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-Delta9-tetrahydrocannabinol (THCA) in whole blood using two-dimensional gas chromatography and EI-mass spectrometry.

机译：使用二维气相色谱和EI质谱法检测全血中的Delta9-四氢大麻酚（THC）和11-nor-9-羧基-Delta9-四氢大麻酚（THCA）。

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摘要

A method is described for the simultaneous analysis of Delta(9)-tetrahydrocannabinol (THC) and its carboxylic acid metabolite, 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THCA) as their trimethylsiyl derivatives using 2-dimensional chromatography and electron ionization-mass spectrometric detection. The addition of a Deans switch to a standard GC oven allows the use of two chromatographic columns of differing stationary phase to greatly reduce matrix interference. The analytes are extracted from 1 mL of whole blood by first precipitating the blood proteins with the addition of acetonitrile followed by solid-phase extraction. The limit of quantitation for both THC and THCA was determined to be 1.0 ng/mL. The between-run precision at 1.0 ng/mL (N = 30) was 7.7% and 7.4% for THC and THCA, respectively. The method is linear from 1 to 100 ng/mL.
机译：描述了一种使用二维色谱法同时分析Delta（9）-四氢大麻酚（THC）及其羧酸代谢物11-nor-9-羧基-Delta（9）-四氢大麻酚（THCA）作为三甲基甲硅烷基衍生物的方法和电子电离质谱法检测。在标准GC柱箱中添加Deans开关后，可以使用两个固定相不同的色谱柱，从而大大降低了基质干扰。首先通过加入乙腈沉淀血液蛋白，然后进行固相萃取，从1 mL全血中提取分析物。 THC和THCA的定量限均确定为1.0 ng / mL。 THC和THCA在1.0 ng / mL（N = 30）时的运行间精密度分别为7.7％和7.4％。该方法从1到100 ng / mL是线性的。

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