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首页> 外文期刊>Journal of Analytical Toxicology >Comparison of an automated and point-of-care immunoassay to GC-MS for urine oxycodone testing in the clinical laboratory.
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Comparison of an automated and point-of-care immunoassay to GC-MS for urine oxycodone testing in the clinical laboratory.

机译：在临床实验室中对尿液中的羟考酮进行自动和即时护理免疫分析与GC-MS的比较。

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OxyContin, a controlled-release formulation of oxycodone, is increasingly abused. Monitoring patient compliance by urine drug testing may deter illegal diversion of OxyContin. Two urine immunoassays were evaluated with a 100 ng/mL cutoff for oxycodone. The Microgenics Corporation Oxycodone DRI on the Bayer ADVIA 1650 and a point-of-care (POC) immunoassay, Monitect Oxycodone POC from Branan Medical Corporation, were compared to gas chromatography-mass spectrometry (GC-MS) with a detection limit of 50 ng/mL free oxycodone. Between-day precision for DRI yielded coefficients of variation from 3.9% to 7.0% at 75 and 125 ng/mL. Fifty-two positive and 52 negative urines were tested. The DRI had a 100% agreement with GC-MS. Two positive specimens had free oxycodone < 50 ng/mL, but oxycodone metabolites, oxymorphone and oxycodone glucuronide > 100 ng/mL, were identified by GC-MS analysis. The POC assay had two false positives and 15 indeterminate (+/-) results. Codeine or hydrocodone was present in all but one of these samples. There was no interference with DRI from morphine, codeine, hydrocodone, hydromorphone, dihydrocodeine, or 6-monoacetyl morphine. Four-hundred and ninety urine samples were subsequently tested with DRI to estimate the oxycodone-positive rate at our hospital, and 47 (9.4%) were positive. The confirmation rate with GC-MS for free oxycodone, not including metabolites, was 93%. The Microgenics DRI offers good performance for oxycodone urine testing and is a better choice for the clinical laboratory than the POC assay. Confirmation of screened positive samples requires a method that can detect total oxycodone and oxymorphone.
机译：羟考酮的控释制剂OxyContin越来越被滥用。通过尿液药物检测监测患者的依从性可能会阻止OxyContin的非法转移。两次尿液免疫分析的羟考酮截断值为100 ng / mL。比较了Bayer ADVIA 1650上的Microgenics Corporation羟考酮DRI和Branan Medical Corporation的即时护理（POC）免疫测定Monitect羟考酮POC与气相色谱-质谱（GC-MS）的检测限为50 ng / mL游离羟考酮。 DRI的日间精度在75和125 ng / mL时产生从3.9％到7.0％的变异系数。测试了52阳性尿和52阴性尿。 DRI与GC-MS达成了100％的协议。两个阳性标本的游离羟考酮含量小于50 ng / mL，但通过GC-MS分析鉴定了羟考酮代谢产物羟吗啡酮和羟考酮葡糖醛酸含量大于100 ng / mL。 POC分析有两个假阳性和15个不确定（+/-）结果。除其中一个样品外，所有样品中均存在可待因或氢可酮。吗啡，可待因，氢可酮，氢吗啡酮，二氢可待因或6一乙酰基吗啡对DRI无干扰。随后用DRI对490份尿液样本进行检测，以评估我院的羟考酮阳性率，其中47例（9.4％）为阳性。用GC-MS对游离羟考酮（不包括代谢物）的确认率为93％。 Microgenics DRI在羟考酮尿液测试中具有良好的性能，是临床实验室比POC测定法更好的选择。筛选阳性样品的确认需要一种可以检测总羟考酮和羟吗啡酮的方法。

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