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首页> 外文期刊>Journal of Analytical Toxicology >Potential surrogate markers for gamma-hydroxybutyrate administration may extend the detection window from 12 to 48 hours.
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Potential surrogate markers for gamma-hydroxybutyrate administration may extend the detection window from 12 to 48 hours.

机译：γ-羟基丁酸酯给药的潜在替代标志物可能会使检测范围从12小时延长到48小时。

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摘要

The exogenous administration of gamma-hydroxybutyrate (GHB) as a drug of abuse, and especially in date rape sexual assaults, has recently increased. Chromatographic techniques are used to detect GHB in blood or urine, with a window of detection limited to 12 h. This brief window makes the proof of administration problematic in most rape cases. This study is aimed to extend the window of detection through surrogate markers of GHB administration. Microarray technology is used in a DBA/2J mouse model to detect gene expression changes in peripheral blood after GHB exposure at times as long as 96 h post exposure. This study focuses on two of the most significantly altered transcripts, epiregulin and phosphoprotein enriched in astrocytes 15 (Pea-15). Both genes have increased the ribonucleic acid expression (8.5- and 4.6-fold upregulation at 96 h, respectively) in GHB-dosed mice (1 g/kg) as compared with the control. To confirm these results at the protein level, an intracellular flow cytometric assay is developed to detect protein level changes in the peripheral blood of both these potential biomarkers after GHB exposure. These results suggest that after further development, epiregulin and Pea-15 may prove to be significant surrogate markers in the indirect detection of GHB administration.
机译：作为一种滥用药物，尤其是在约会强奸性攻击中，γ-羟基丁酸酯（GHB）的外源给药最近有所增加。色谱技术用于检测血液或尿液中的GHB，检测时间限制为12小时。这个简短的窗口使行政证明在大多数强奸案中成问题。这项研究旨在通过GHB给药的替代标志物扩大检测范围。在DBA / 2J小鼠模型中使用微阵列技术来检测GHB暴露后长达96小时后外周血中基因表达的变化。这项研究的重点是星形胶质细胞15（Pea-15）中最显着改变的两个转录本，即上皮调节蛋白和磷酸化蛋白。与对照相比，两种基因均增加了GHB剂量小鼠（1 g / kg）的核糖核酸表达（分别在96 h时分别为8.5和4.6倍上调）。为了在蛋白质水平上确认这些结果，开发了一种细胞内流式细胞术测定法，以检测GHB暴露后这两种潜在生物标志物外周血中蛋白质水平的变化。这些结果表明，在进一步发展之后，上调蛋白和Pea-15可能被证明是间接检测GHB的重要标志物。

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