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首页> 外文期刊>Journal of Analytical Toxicology >Ofloxacin as a reference marker in hair of various colors.
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Ofloxacin as a reference marker in hair of various colors.

机译：氧氟沙星作为各种颜色头发中的参考标记。

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It has been proposed that administration of a reliable marker substance to human subjects may enhance the ability to identify drug use and treatment compliance in drug treatment programs. The goal of this study was to determine if an oral dose of the antibiotic ofloxacin (OFLX) could be used as a "marker" substance to establish reference points with respect to time in hair of various colors. Male and female subjects (n = 32) between 18 and 40 years of age received 800 mg of OFLX as a divided oral dose on a single day. Subjects were restricted from cutting their hair or performing chemical treatments. Hair was collected (by cutting) before, and at weeks 4, 5, 6, and 7 after drug administration. Subjects were classified as having black (n = 5), brown (n = 13), blonde (n = 8), or red (n = 6) hair. Hair was segmented into 3.0-cm segments prior to digestion, extraction, and analysis by high-pressure liquid chromatography (HPLC). At 7 weeks, the mean OLFX concentrations (+/- 1 SD) in the first 3.0 cm of hairclosest to the scalp were as follows: 30.6 +/- 8.5 ng/mg (black), 6.0 +/- 1.8 ng/mg (brown), 3.5 +/- 1.6 ng/mg (blonde), and 1.4 +/- 0.3 ng/mg (red). A similar pattern was found in hair collected at weeks 4-6. Quantitative eumelanin (EUM) hair concentrations for each subject were also determined for each subject via HPLC. A strong relationship between OFLX concentration at 7 weeks and EUM was noted (r2 adjusted = 0.728; p < 0.001). In six subjects, we also determined the intrasubject variability of OFLX incorporation into individual hair strands. Four strands from each subject were segmented into 2-mm segments and analyzed. OFLX appeared in segments #1-#10 at week 5 (the first centimeter of hair). OFLX appeared in segments #2-#20 at week 7 (the first and second centimeter of hair). The maximum OFLX concentration (the "band" of drug) and location was then determined for each strand. The maximum OFLX concentration was measured in segments #2-#5 at week 5 for all subjects (within the first centimeter of hair length). The maximum OFLX concentration was measured in segments #3-#8 at week 7 (within the first and second centimeter of hair). This was consistent with a growth rate of less than 1.0 cm/month, although considerable intersubject variability was found. No significant axial diffusion of OFLX along the hair shaft beyond the first 3.0 cm of hair was noted. Despite a strong effect of hair color, these data suggest that OFLX may be a suitable marker substance for hair, allowing a subject to serve as their own "control". Future studies will explore whether drug use, treatment compliance, or recidivism in clinical drug-abuse studies can be determined with the aid of OFLX.
机译：已经提出对人对象施用可靠的标记物质可以增强在药物治疗计划中鉴定药物使用和治疗依从性的能力。这项研究的目的是确定口服剂量的氧氟沙星（OFLX）抗生素是否可以用作“标记”物质，以建立各种颜色的头发相对于时间的参考点。年龄在18至40岁之间的男性和女性受试者（n = 32）在一天中分别接受800 mg OFLX口服。限制受试者剪头发或进行化学治疗。在给药前和给药后第4、5、6和7周收集头发（通过剪发）。受试者被分类为黑色（n = 5），棕色（n = 13），金发（n = 8）或红色（n = 6）头发。在进行消化，提取和高压液相色谱（HPLC）分析之前，将头发切成3.0厘米的片段。在第7周，最靠近头皮的前3.0 cm头发的平均OLFX浓度（+/- 1 SD）如下：30.6 +/- 8.5 ng / mg（黑色），6.0 +/- 1.8 ng / mg（黑色）棕色），3.5 +/- 1.6 ng / mg（红色）和1.4 +/- 0.3 ng / mg（红色）。在第4-6周收集的头发中发现了类似的模式。还通过HPLC为每个受试者确定了每个受试者的定量Eumelanin（EUM）头发浓度。注意到在7周时OFLX浓度与EUM之间有很强的关系（r2调整为0.728; p <0.001）。在六名受试者中，我们还确定了将OFLX纳入个体发束的受试者内部变异性。将来自每个受试者的四股线切成2毫米的片段并进行分析。 OFLX在第5周（头发的第一厘米）出现在＃1-＃10段中。 OFLX出现在第7周（头发的第一和第二厘米）的＃2-＃20段中。然后确定每条链的最大OFLX浓度（药物的“条带”）和位置。在第5周的所有受试者（头发长度的第一厘米以内），在＃2-＃5段中测量了最大OFLX浓度。在第7周（在头发的第一和第二厘米内）在＃3-＃8段中测量最大OFLX浓度。尽管发现受试者之间存在相当大的变异性，但这与小于1.0厘米/月的生长速度是一致的。没有观察到OFLX沿着发干的明显轴向扩散超过最初的3.0 cm。尽管染发效果很强，但这些数据表明OFLX可能是适合染发的标记物质，可让受试者充当自己的“对照”。未来的研究将探索是否可以借助OFLX来确定临床药物滥用研究中的药物使用，治疗依从性或累犯性。

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