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首页> 外文期刊>Journal of Anatomy >In vivo fibreoptic confocal imaging (FOCI) of the human ocular surface.
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In vivo fibreoptic confocal imaging (FOCI) of the human ocular surface.

机译：人眼表面的体内纤维共聚焦成像（FOCI）。

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Recent developments in the miniaturization of confocal imaging technology have resulted in the development of a hand-held confocal microscope probe. There are many structures of interest in the human eye that are within reach of a fluorescence-mode confocal microscope; this study assessed the feasibility of in vivo human ocular imaging. Safety analysis was undertaken to ensure that the laser light applied to the ocular surface structures constituted no threat to patient safety. A fibreoptic confocal imaging (FOCI) probe using an illumination wavelength of 488 nm was applied to the ocular surface of four volunteers after topical administration of sodium fluorescein. Stabilization of the probe on the ocular surface was difficult, but movement artefacts could be minimized to a satisfactory level in most subjects by a variety of procedures. High-quality images of conjunctival epithelial and goblet cells, lamina propria structures, accessory lacrimal glands, lacrimal ducts and superficial sclera were obtained. Lateral resolution was 1-1.5 microm and axial resolution was approximately 30 microm; individual erythrocytes could be seen in conjunctival vessels. The rete ridges and intervening epithelial components, including the probable location of corneal limbal stem cells, could be viewed, although it was not possible to distinguish cell subgroups. The study showed that fluorescence-mode imaging of the ocular surface is a viable and promising tool for assessment of diseases and processes involving superficial ocular structures. Refinement of equipment and techniques, particularly probe stabilization, is necessary to realize fully the potential of FOCI for ocular use.
机译：共焦成像技术的小型化的最新发展导致了手持共焦显微镜探针的发展。人眼中有许多感兴趣的结构都在荧光模式共聚焦显微镜的范围内。这项研究评估了体内人眼成像的可行性。进行了安全分析，以确保施加到眼表结构的激光不会对患者的安全构成威胁。在局部施用荧光素钠后，将使用488 nm照明波长的光纤共聚焦成像（FOCI）探针应用于四名志愿者的眼表。很难将探针稳定在眼表面上，但是可以通过多种方法将运动伪影最小化到大多数受试者的满意水平。获得了结膜上皮和杯状细胞，固有层结构，副泪腺，泪管和浅表巩膜的高质量图像。横向分辨率为1-1.5微米，轴向分辨率约为30微米;结膜血管中可见单个红细胞。尽管无法区分细胞亚群，但可以观察到网纹和中间上皮成分，包括角膜缘干细胞的可能位置。研究表明，眼表的荧光模式成像是评估涉及浅表眼结构的疾病和过程的可行且有前途的工具。要充分利用FOCI在眼科方面的潜力，必须改进设备和技术，尤其是稳定探头。

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