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首页> 外文期刊>Journal of Andrology >Castration- and aging-induced changes in the expression of zinc transporter and metallothionein in rat prostate.
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Castration- and aging-induced changes in the expression of zinc transporter and metallothionein in rat prostate.

机译：去势和衰老诱导的大鼠前列腺锌转运蛋白和金属硫蛋白表达的变化。

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摘要

Prostate tissue contains high concentrations of zinc. Zinc content in the prostate gland changes in prostatic disease, such as benign prostate hyperplasia and prostate cancer, which occur more frequently with increasing age. Prostate zinc content is also known to decrease after castration in animal models. It is not clear how prostate zinc content is regulated; therefore, to clarify the mechanisms underlying zinc homeostasis, we examined zinc content and the expression of zinc transporters and metallothioneins in the prostates of aged or castrated rats. Zinc concentration was measured by flame atomic absorption spectrometry. The mRNA expression of zinc transporters and metallothioneins was determined by real-time reverse transcriptase polymerase chain reaction analysis. The expression of the zinc transporter Slc30a2 (Znt2) in ventral prostate (VP) of aged rats (21 months) was approximately 21-fold higher than that in VP of young rats (4 months), and zinc levels in VP of young rats increased significantly compared with that in aged rats. Zinc content in lateral prostate (LP) and dorsal prostate did not differ between young and aged rats. Decreased metallothionein-3 (Mt3) expression was observed in LP of castrated rats, and this reduction was prevented by testosterone replacement. Zinc content and Mt3 expression levels correlated significantly in rat LP. Our findings suggest that Mt3 could play a critical role in zinc homeostasis in rat LP.
机译：前列腺组织中含有高浓度的锌。前列腺疾病中前列腺中锌的含量发生变化，例如良性前列腺增生和前列腺癌，随着年龄的增长，前列腺癌中的锌含量会更高。在动物模型中，去势后前列腺锌含量也会降低。目前尚不清楚如何调节前列腺锌含量。因此，为阐明锌体内稳态的机制，我们检查了锌含量以及衰老或去势大鼠前列腺中锌转运蛋白和金属硫蛋白的表达。锌浓度通过火焰原子吸收光谱法测量。通过实时逆转录酶聚合酶链反应分析确定锌转运蛋白和金属硫蛋白的mRNA表达。锌转运蛋白Slc30a2（Znt2）在老年大鼠（21个月）的腹侧前列腺（VP）中的表达比年轻大鼠（4个月）的VP约高21倍，并且年轻大鼠VP中的锌水平升高与老年大鼠相比有显着性差异。幼年和老年大鼠的外侧前列腺（LP）和背侧前列腺中的锌含量没有差异。在去势大鼠的LP中观察到金属硫蛋白3（Mt3）表达降低，并且这种降低可以通过睾丸激素替代来预防。大鼠LP中锌含量和Mt3表达水平显着相关。我们的发现表明，Mt3可能在大鼠LP的锌稳态中起关键作用。

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