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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Determination of sulfamethazine and trimethoprim in liquid feed premixes by HPLC and diode array detection, with an analysis of the uncertainty of the analytical results.
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Determination of sulfamethazine and trimethoprim in liquid feed premixes by HPLC and diode array detection, with an analysis of the uncertainty of the analytical results.

机译：高效液相色谱法和二极管阵列检测法测定液体饲料预混合物中的磺胺二甲嘧啶和甲氧苄啶，并分析了分析结果的不确定性。

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摘要

Sulfamethazine (SMZ) and trimethoprim (TMP) are antibacterials used in veterinary practice. This paper describes a method for their determination in veterinary liquid feed premixes that is based on liquid chromatography with diode array detection. Gradient elution with methanol and ammonium acetate achieved excellent separation of the two analytes within 15 min without any interference from the matrix. Absorbance of the column effluent was monitored at 264 nm for SMZ and at 230 nm for TMP. Detailed analyses of the uncertainties of determinations afford estimated expanded uncertainties of, respectively, 0.2 and 0.1 w/v % for typical SMZ and TMP concentrations of 10.7 and 2.1 w/v %, respectively. At the lower end of the calibrated range of the method, the dominant source of uncertainty is the preparation of standards and the construction of the calibration line.
机译：磺胺二甲嘧啶（SMZ）和甲氧苄啶（TMP）是兽医实践中使用的抗菌剂。本文介绍了一种基于液相色谱和二极管阵列检测的兽用液体饲料预混物中含量测定的方法。用甲醇和乙酸铵进行梯度洗脱可在15分钟内实现两种分析物的出色分离，而不受基质的干扰。对于SMZ，在264 nm处监测色谱柱流出物的吸光度;对于TMP，在230 nm处监测色谱柱流出物的吸光度。对测定不确定度的详细分析提供了典型SMZ和TMP浓度分别为10.7和2.1 w / v％的估计不确定度，分别为0.2和0.1 w / v％。在该方法的校准范围的下限，不确定性的主要来源是标准液的制备和校准线的构建。

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