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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Antioxidant enzymes and DPPH-Radical scavenging activity in chilled and heat-shocked rice (Oryza sativa L.) seedlings radicles
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Antioxidant enzymes and DPPH-Radical scavenging activity in chilled and heat-shocked rice (Oryza sativa L.) seedlings radicles

机译:冷热激水稻幼苗胚根的抗氧化酶和DP​​PH清除自由基的活性

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摘要

Chilling whole rice seedlings at 5 degreesC significantly increased the time needed to recover linear growth and reduced the subsequent linear rate of radicle growth. Subjecting nonchilled seedlings to a 45 degreesC heat shook for up to 20 min did not alter subsequent growth, whereas a 3 min heat shock was optimal in reducing growth inhibition caused by 2 days of chilling. The activity of five antioxidant enzymes [superoxide dismutase (EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), glutathione reductase (GR; EC 1.6.4.2), and guaiacol peroxidase (GPX; EC 1.11.1.7)] and DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity were measured in heat-shocked and/or chilled radicles. Heat shock slightly increased the activity of CAT, APX, and GR and suppressed the increase of GR and GPX activity during recovery from chilling. Increased CAT, APX, GR, and DPPH-radical scavenging activity and protection of CAT activity during chilling appear to be correlated with heat shock-induced chilling tolerance.
机译:在5°C下冷冻全米幼苗会显着增加恢复线性生长所需的时间,并降低随后的胚根生长线性速率。将未冷藏的幼苗在45°C的温度下摇晃20分钟不会改变随后的生长,而3分钟的热休克对于减少2天的冷藏引起的生长抑制作用是最佳的。五个抗氧化酶的活性[超氧化物歧化酶(EC 1.15.1.1),过氧化氢酶(CAT; EC 1.11.1.6),抗坏血酸过氧化物酶(APX; EC 1.11.1.11),谷胱甘肽还原酶(GR; EC 1.6.4.2)和愈创木酚在热激和/或冷却的胚根中测定了过氧化物酶(GPX; EC 1.11.1.7)和DPPH(1,1-二苯基-2-吡啶并肼基)的自由基清除活性。热激稍微增加了CAT,APX和GR的活性,并抑制了从冷却中恢复期间GR和GPX活性的增加。 CAT,APX,GR和DPPH自由基清除活性的增加以及在冷却过程中对CAT活性的保护似乎与热激诱导的耐寒性相关。

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