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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Rapid fluorescence screening assay for enrofloxacin and tetracyclines in chicken muscle.
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Rapid fluorescence screening assay for enrofloxacin and tetracyclines in chicken muscle.

机译：鸡肌肉中恩诺沙星和四环素的快速荧光筛选测定。

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摘要

A simple, rapid fluorescence assay was developed for screening both enrofloxacin (ENRO) and tetracyclines in chicken muscle at the U.S. tolerance levels (300 ng/g and 2 mug/g, respectively). Screening for both classes of antibiotics is accomplished using one extraction, thus simplifying and expediting the process. The method requires an initial extraction of chicken muscle with 1% acetic acid in acetonitrile, centrifugation, and analysis of the supernatant for ENRO fluorescence. After addition of ammonium hydroxide, magnesium chloride, and methanol, followed by centrifugation and filtration, the supernatant can be measured for tetracycline fluorescence. Chlortetracycline (CTC) was chosen as a representative tetracycline to demonstrate the method, as it displays intermediate sensitivity among the three tetracyclines approved in the U.S. Comparison of the fluorescence of control and tolerance-level-fortified samples of both ENRO and CTC shows no overlap. Setting a threshold as the average fortified fluorescence minus 3sigma allows for successful screening, as illustrated with blind samples as controls or fortified with ENRO and/or CTC over a range of concentrations. This method can provide an alternative or supplemental approach to currently used microbial screening assays.
机译：开发了一种简单，快速的荧光测定法，用于以美国耐受水平（分别为300 ng / g和2杯/ g）筛查鸡肉中的恩诺沙星（ENRO）和四环素。使用一次提取即可完成对两种抗生素的筛选，从而简化并加快了这一过程。该方法需要先用1％乙腈中的乙酸提取鸡肌肉，离心并分析上清液的ENRO荧光。加入氢氧化铵，氯化镁和甲醇后，离心和过滤，可以测量上清液的四环素荧光。选择金霉素（CTC）作为具有代表性的四环素来证明该方法，因为它在美国批准的三种四环素中显示出中等灵敏度.ENRO和CTC的对照和强化水平样品的荧光比较没有重叠。将阈值设置为平均强化荧光值减去3sigma可以成功筛选，如以盲样品作为对照或在一定浓度范围内用ENRO和/或CTC进行强化。该方法可以为当前使用的微生物筛选测定提供替代或补充的方法。

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