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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Cellulose-Based Chromatography for Cellooligosaccharide Production
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Cellulose-Based Chromatography for Cellooligosaccharide Production

机译：基于纤维素的层析法生产纤维寡糖

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摘要

The potential of using cellulose stationary phases for the chromatographic fractionation of cellooli-gosaccharide preparations has been explored.The impetus for the work is the current interest in using cellooligosaccharides as functional nondigestible oligosaccharides in foods.The conceptual studies illustrate the potential of using ethanol-water mobile phases in conjunction with cellulose stationary phases for Cellooligosaccharide fractionation.Cellooligosaccharide solubility in ethanol-water mixtures and their elution order from cellulose-based columns using ethanol-water mobile phases were shown to be in line with their degree of polymerization (DP),with the higher DP cellooligosaccharides being less soluble and having longer retention times.The retention volume for all COS increased with increased temperature.Both microcrystalline and fibrous cellulose preparations were shown to work as chromatographic stationary phases.The application experiments demonstrate the potential of using cellulose stationary phases for the cleanup and fractionation of Cellooligosaccharide mixtures generated via acid-catalyzed hydrolysis of cellulose.
机译：已经探索了使用纤维素固定相进行纤维素低聚糖制剂色谱分离的潜力。这项工作的推动力是目前将纤维素低聚糖用作食品中功能性非消化性低聚糖的兴趣。概念研究表明了使用乙醇-水的潜力流动相与纤维素固定相一起用于纤维素低聚糖的分离。纤维素低聚糖在乙醇-水混合物中的溶解度以及使用乙醇-水流动相从纤维素基色谱柱上的洗脱顺序显示与它们的聚合度（DP）一致。较高的DP纤维寡糖溶解度较低，并且保留时间更长。所有COS的保留体积均随温度的升高而增加。微晶纤维和纤维素纤维制品均表现出色谱固定相的作用。应用实验证明了usin的潜力g纤维素固定相，用于纯化和分级分离通过酸催化水解纤维素产生的纤维寡糖混合物。

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