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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Assessment of Carotenoid Bioavailability of Whole Foods Using a Caco-2 Cell Culture Model Coupled with an in Vitro Digestion
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Assessment of Carotenoid Bioavailability of Whole Foods Using a Caco-2 Cell Culture Model Coupled with an in Vitro Digestion

机译：使用Caco-2细胞培养模型结合体外消化评估整个食物中类胡萝卜素的生物利用度

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Epidemiological studies have shown that consumption of carotenoid-rich fruits and vegetables is associated with a reduced risk of developing chronic diseases.beta-Carotene,a-carotene,and beta-cryptoxanthin are precursors of vitamin A,a nutrient essential for human health.However,little is known about the bioavailability of carotenoids from whole foods.This study characterized the intestinal uptake performance of carotenoids using monolayers of differentiated Caco-2 human intestinal cells and mimicked human digestion to assess carotenoid absorption from carrots and corn.Results showed that Caco-2 cellular uptake of beta-carotene and zeaxanthin was higher than that of lutein.Uptake performances of pure carotenoids and carotenoids from whole foods by Caco-2 cells were both curvilinear,reaching saturated levels after 4 h of incubation.The time kinetics and dose response of carotenoid uptake presented a similar pattern in Caco-2 cells after plating for 2 and 14 days.Furthermore,the applicability of this new model was verified with whole grain corn,showing that cooked corn grain significantly enhanced carotenoid bioavailability.These results support the feasibility of the in vitro digestion cell model for assessing carotenoid absorption from whole foods as a suitable and cost-effective physiological alternative to current methodologies.
机译：流行病学研究表明，食用富含类胡萝卜素的水果和蔬菜与减少患上慢性病的风险有关.β-胡萝卜素，α-胡萝卜素和β-隐黄质是维生素A的前体，维生素A是人类健康必不可少的营养素。整个食品中类胡萝卜素的生物利用度鲜为人知。这项研究利用分化的Caco-2人肠道细胞单层膜并通过模拟人体消化来评估胡萝卜和玉米中类胡萝卜素的吸收来表征类胡萝卜素的肠道吸收性能。结果表明，Caco- 2细胞对β-胡萝卜素和玉米黄质的吸收高于叶黄素。Caco-2细胞从纯食品中摄取的纯类胡萝卜素和类胡萝卜素的曲线均呈曲线，孵育4小时后达到饱和水平。时间动力学和剂量响应接种2天和14天后，Caco-2细胞中类胡萝卜素的摄取呈现相似的模式。全谷物玉米验证了该新模型的适应性，表明煮熟的玉米谷物显着提高了类胡萝卜素的生物利用度。这些结果支持体外消化细胞模型评估全食类胡萝卜素吸收作为可行性和成本效益的生理替代品的可行性。到目前的方法。

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