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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Isolation and characterization of defatted canola meal protein.
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Isolation and characterization of defatted canola meal protein.

机译：脱脂菜籽粕蛋白的分离和鉴定。

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More than 99% of protein was extracted from crude commercial hexane defatted canola [rape] meal when a 5% w/v suspension in 0.4% w/v NaOH was agitated for 60 min at room temperature in baffled flasks on an orbital shaker at 180-200 rpm. Protein recovery was 87.5% upon precipitation with acetic acid. The major proteins in canola seed were glutelins (91.8% soluble in 0.4% w/v NaOH) and globulins (50.9% soluble in 5% w/v NaCl) as measured by separate extractions. Canola proteins had poor solubility between pH 2 and 10 for all dispersion solutions. Solubility of protein isolates was 60% or less. Extraction and precipitation procedure did not produce lysinoalanine. Amino acid profile of isolates indicated high nutritional quality for use in products for 10-12-year-olds and adults.
机译：在室温下于180℃的折流烧瓶中，在装有挡板的烧瓶中于室温下搅拌5％w / v的0.4％w / v NaOH悬浮液，从粗商业己烷脱脂的低芥酸菜籽[强奸]粗粉中提取了超过99％的蛋白质。 -200 rpm。用乙酸沉淀后，蛋白质回收率为87.5％。通过分别提取测定，双低油菜籽中的主要蛋白质是谷蛋白（91.8％溶于0.4％w / v NaOH）和球蛋白（50.9％溶于5％w / v NaCl）。对于所有分散液，低芥酸菜子蛋白质在pH 2和10之间的溶解度都很差。蛋白质分离物的溶解度为60％或更低。提取和沉淀过程未产生赖氨酸丙氨酸。分离物的氨基酸谱表明，该营养品适用于10-12岁的成年人和成年人。

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