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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Zinc transport in Caco-2 cells and zinc balance in rats: influence of the heat treatment of a casein-glucose-fructose mixture.
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Zinc transport in Caco-2 cells and zinc balance in rats: influence of the heat treatment of a casein-glucose-fructose mixture.

机译：锌在Caco-2细胞中的转运和大鼠中的锌平衡：酪蛋白-葡萄糖-果糖混合物热处理的影响。

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摘要

The effects of the heat treatment of casein in the presence of glucose-fructose on Zn bioavailability were studied. Changes in Zn speciation were compared after in vitro digestion of heated (HC) and unheated mixture (C) alone and as part of the diet. The uptake and transport of digested soluble Zn was investigated in Caco-2 cells grown in bicameral chambers; balance studies were done in rats fed diets containing the different samples. After in vitro digestion, the precipitated Zn was significantly higher in HC than in C. In assays with Caco-2 cells, the amount of Zn transferred from the apical to the basolateral chamber was significantly greater when the culture medium contained raw or heated casein. However, because a larger proportion of Zn was precipitated by in vitro digestion, Zn utilization was less efficient in the presence of casein. In biological experiments, food efficiency of the heated casein-glucose-fructose diet was lower, and feeding this diet increased the urinary Zn excretion and lowered Zn absorption and retention. The effects of browning products generated during food processing should be taken into account, especially in diets containing marginally adequate levels of Zn, to prevent possible deficiency.
机译：研究了葡萄糖果糖存在下酪蛋白热处理对锌生物利用度的影响。单独和作为饮食的一部分对加热的（HC）和不加热的混合物（C）进行体外消化后，比较了锌的形态变化。研究了在双房室中生长的Caco-2细胞中消化的可溶性锌的吸收和转运。在饲喂含有不同样品的饮食的大鼠中进行了平衡研究。体外消化后，HC中沉淀的Zn显着高于C中的Zn。在使用Caco-2细胞的分析中，当培养基中含有未加工或加热的酪蛋白时，从根尖转移至基底外侧腔室的Zn量显着增加。但是，由于大量的锌通过体外消化沉淀，因此在酪蛋白存在下锌的利用效率较低。在生物学实验中，加热的酪蛋白-葡萄糖-果糖饮食的食物效率较低，而饲喂该饮食会增加尿中锌的排泄，并降低锌的吸收和保留。应考虑到在食品加工过程中产生的褐变产物的影响，尤其是在含少量锌的日粮中，以防止可能的缺乏。

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