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首页> 外文期刊>Journal of applied biological chemistry >In vitro Antimutagenic and Genotoxic Effects of Azadirachta indica Extract
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In vitro Antimutagenic and Genotoxic Effects of Azadirachta indica Extract

机译：印za提取物的体外抗诱变和遗传毒性作用

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Azadirachta indica extract (ATE) has been regarded as a promising source of environment-friendly organic materials owing to their low mammalian toxicity. However, quite a bit of research has been reported that AIE may cause clastogens in human lymphocytes. Therefore, this study was conducted to evaluate the antimutagenic and genotoxicity of two samples of AIE. Antimutagenic test was experimented by using bacterial reverse mutation test. In the bacterial reverse mutation test, five strains Salmonella Typhimurim of two samples of AIE in order to evaluate its mutagenic potential. Bacterial reverse mutation test was also performed on positive control and negative control groups in the presence of the metabolic activation system (S-9 mix) and metabolic non-activation system. In the chromosome aberration test, Chinese hamster lung cells were exposed to ATE for 6 or 24 h with BPS, or for 6 h with S-9 mix. Negative and positive control groups were experimented for chromosome aberration test. As a result, the number of mutated colonies induced by 4-NQO were reduced by ATE treatment in all strains, indicating that ATE may have antimutagenic effects. Bacterial reverse mutation and chromosomal aberration were not shown at all concentration of AIE, regardless of activation of the metabolic system. we concluded that two AIE samples used in this study have no genotoxic effects to human, according to the genotoxicity battery system suggested by ICH (International Conference on Harmonization).
机译：印za提取物（ATE）由于其对哺乳动物的低毒性而被认为是一种有前途的环保有机材料来源。然而，已经有相当多的研究报道了AIE可能会在人类淋巴细胞中引起杀伤原。因此，本研究旨在评估两个AIE样品的抗诱变性和遗传毒性。采用细菌反向突变试验进行抗突变试验。在细菌反向突变测试中，对两个AIE样品的五株鼠伤寒沙门氏菌进行了评估，以评估其诱变潜力。在存在代谢激活系统（S-9混合物）和代谢非激活系统的情况下，还对阳性对照组和阴性对照组进行了细菌逆向突变测试。在染色体畸变测试中，中国仓鼠肺细胞用BPS暴露于ATE 6或24小时，或使用S-9混合物暴露6个小时。阴性和阳性对照组进行染色体畸变测试。结果，在所有菌株中，通过ATE处理减少了由4-NQO诱导的突变菌落的数量，表明ATE可能具有抗诱变作用。不论代谢系统的激活如何，在所有AIE浓度下均未显示出细菌反向突变和染色体畸变。我们得出的结论是，根据ICH（国际协调会议）建议的遗传毒性电池系统，本研究中使用的两个AIE样品对人体没有遗传毒性作用。

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