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首页> 外文期刊>Journal of Applied Bacteriology >STRAIN-SPECIFIC FINGERPRINTS OF RHIZOBIUM GALEGAE GENERATED BY PCR WITH ARBITRARY AND REPETITIVE PRIMERS
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STRAIN-SPECIFIC FINGERPRINTS OF RHIZOBIUM GALEGAE GENERATED BY PCR WITH ARBITRARY AND REPETITIVE PRIMERS

机译：任意主位和重复主位PCR PCR产生的加利氏根瘤菌的应变指纹图谱

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摘要

Strain-specific genomic patterns of Rhizobium galegae were generated by PCR using both arbitrary and repetitive (BOX, ERIC and REP) primers. The identification of the strains was achieved also by RFLP analysis. However, the PCR genomic fingerprinting has significant advantages: it is not only simpler and faster, but it is also much more discriminative because it deals with the full bacterial genome and not only with parts of it as is the case with RFLP. In addition, both kinds of PCR fingerprinting (using arbitrary or repetitive primers) generated highly specific and reproducible patterns when parallel reactions with total bacterial DNA, extracted from independent liquid cultures were performed. The latter shows that AP- and rep-PCR are convenient for controlling the production and application of Rhizobium inoculants.
机译：利用任意和重复的（BOX，ERIC和REP）引物通过PCR生成半球形根瘤菌的菌株特异性基因组模式。菌株的鉴定也通过RFLP分析实现。但是，PCR基因组指纹图谱具有显着的优势：它不仅更简单，更快速，而且更具判别性，因为它可以处理完整的细菌基因组，并且不仅可以处理部分细菌基因组（与RFLP一样）。另外，当与从独立液体培养物中提取的总细菌DNA进行平行反应时，两种PCR指纹图谱（使用任意或重复的引物）均产生高度特异性和可重复的模式。后者表明AP-和rep-PCR方便用于控制根瘤菌接种剂的生产和应用。

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