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首页> 外文期刊>Journal of Applied Bacteriology >EFFECT OF PRE- AND POST-HEAT SHOCK TEMPERATURE ON THE PERSISTENCE OF THERMOTOLERANCE AND HEAT SHOCK-INDUCED PROTEINS IN LISTERIA MONOCYTOGENES
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EFFECT OF PRE- AND POST-HEAT SHOCK TEMPERATURE ON THE PERSISTENCE OF THERMOTOLERANCE AND HEAT SHOCK-INDUCED PROTEINS IN LISTERIA MONOCYTOGENES

机译：热震前后的温度对荔枝单胞菌热耐受性和热震诱导蛋白持久性的影响

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摘要

The effect of incubation temperature, before and after a heat shock, on thermotolerance of Listeria monocytogenes at 58 degrees C was investigated. Exposing cells grown at 10 degrees C and 30 degrees C to a heat shock resulted in similar rises in thermotolerance while the increase was significantly higher when cells were grown at 4 degrees C prior to the heat shock. Cells held at 4 degrees C and 10 degrees C after heat shock maintained heat shock-induced thermotolerance for longer than cells held at 30 degrees C. The growth temperature prier to inactivation had negligible effect on the persistence of heat shock-induced thermotolerance. Concurrent with measurements of thermotolerance were measurements of the levels of heat shock-induced proteins. Major proteins showing increased synthesis upon the heat shock had approximate molecular weights of 84, 74, 63, 25 and 19 kDa. There was little correlation between the loss of thermotolerance after the heat shock and the levels of these proteins. Thermotolerance of heat shocked and non-heat shocked cells was described by traditional log-linear kinetics and a model describing a sigmoidal death curve (logistic model). Employing log-linear kinetics resulted in a poor fit to a major part of the data whereas a good fit was achieved by the use of a logistic model.
机译：研究了热休克前后热温对单核细胞增生李斯特菌在58摄氏度下耐热性的影响。将在10摄氏度和30摄氏度下生长的细胞暴露于热冲击下会导致相似的耐热性升高，而当细胞在热激之前在4摄氏度下生长时，其耐热性会明显提高。热休克后保持在4摄氏度和10摄氏度的细胞保持热休克诱导的热耐受性的时间比保持30摄氏度的细胞保持更长的时间。失活的生长温度对热休克诱导的热耐受性的持久性影响可忽略不计。与耐热性的测量同时是对热激诱导的蛋白质水平的测量。在热激后显示增加的合成的主要蛋白质具有大约84、74、63、25和19 kDa的分子量。热冲击后耐热性的丧失与这些蛋白质水平之间几乎没有相关性。通过传统的对数线性动力学和描述S形死亡曲线的模型（逻辑模型）描述了热激和非热激细胞的耐热性。采用对数线性动力学会导致对大部分数据的拟合度较差，而采用逻辑模型则拟合效果良好。

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