• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of applied biological chemistry >Production of Transgenic Plants in Brassica napus Winter Cultivar 'Youngsan'
【24h】

Production of Transgenic Plants in Brassica napus Winter Cultivar 'Youngsan'

机译：甘蓝型油菜冬季品种“ Youngsan”的转基因植物生产

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To improve genetic transformation of Brassica napus winter cultivar 'Youngsan', factors influencing shoot regeneration and transformation from cotyledonary petioles were investigated. Shoot induction was enhanced in the combination of 0.5 mg/L NAA and2-4 mg/L kinetin. Silver nitrate was essential for successful shoot regeneration, ranging from 5 to 9 mg/L. The addition of GA_3 promoted plant regeneration. Among the tested Agrobacterium strains, co-cultivation times, and antibiotic selection regimes,choice of appropriate Agrobacterium strain was the most critical factor for efficient transformation of B. napus cv. 'Youngsan'. The EHA105 succinamopine strain was the most efficient and the maximum transformation efficiency was 26.8%. Transgenic shoots were selected on 10 mg/L phosphinothricin (PPT) containing media. The transgenic plants expressing bar and gus genes were resistant for commercial herbicide "Basta" and stained with X-Gluc. Southern blot hybridization indicated that the presence of oneto three gus gene copies per genome and inheritance of the gus gene into the Ti generation.
机译：为了提高甘蓝型油菜冬季品种“ Youngsan”的遗传转化，研究了影响子叶柄芽再生和转化的因素。 0.5 mg / L NAA和2-4 mg / L激动素的组合可增强芽诱导。硝酸银对于成功地再生芽是必不可少的，其范围为5至9 mg / L。 GA_3的添加促进了植物的再生。在测试的农杆菌菌株，共培养时间和抗生素选择方案中，选择合适的农杆菌菌株是有效转化油菜双歧杆菌的最关键因素。 'Youngsan'。 EHA105琥珀氨平菌株效率最高，最大转化效率为26.8％。在含10 mg / L膦丝菌素（PPT）的培养基上选择转基因芽。表达bar和gus基因的转基因植物对商业除草剂“ Basta”具有抗性，并用X-Gluc染色。 Southern印迹杂交表明，每个基因组存在一个至三个gus基因拷贝，并且该gus基因遗传到Ti世代中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号