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首页> 外文期刊>Journal of Aquaculture in the Tropics >GENERATION OF DNA BARCODE DATA BASE FOR GUNTHERS LOACH, NEMACHEILUS GUENTHERI (DAY, 1867)
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GENERATION OF DNA BARCODE DATA BASE FOR GUNTHERS LOACH, NEMACHEILUS GUENTHERI (DAY, 1867)

机译：冈萨斯州内马切伊勒斯（NEMACHEILUS GUENTHERI）枪手泥ACH的DNA条码数据库的生成（1867年，星期）

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摘要

The Gunther's loach, Nemacheilus guentheri (Day, 1867) was collected from the streams of Harangi river, Kalur village, Madikeri District of Karnataka State. Morphometric and meristic characterization was carried out for N. guentheri. The DNA was extracted from N. guentheriwith both phenol chloroform method and HiPurA multi-Sample DNA purification kit. Purity of DNA was checked with UV spectrophotometer (260 nm and 280nm) and DNA was confirmed with Agarose gel Electrophoresis (1%). DNA barcoding was carried out for the DNA extracts using Cytochrome c Oxidase subunit 1(COI) gene. Polymerase Chain Reaction (PCR) was carried with COI gene primer and the PCR product size of 652bp was confirmed with Agarose gel electrophoresis (2%) using 100bp DNA ladder andDNA content was analyzed with Biophotometer (Eppendorf). The purity and concentration of DNA was determined at 260nm and 280 nm, the purity and concentration recorded for the PCR product were 1.58-1.63 (DNA quality is good) and 7.12 (4.6-10.1) respectively. PCR products of COI gene were sequenced and submitted to Genbank (NCBI), the accession numbers were provided by GenBank for N. guentheri includes KP772692, KP772693, KP772694 and KP772695.
机译：冈瑟的泥ach Nemacheilus guentheri（Day，1867年）是从卡纳塔克邦马蒂科里区Kalur村的Harangi河溪流中采集的。形态学和meristic表征进行了N. guentheri。用酚氯仿法和HiPurA多样品DNA纯化试剂盒从瓜氏猪笼草提取DNA。用UV分光光度计（260nm和280nm）检查DNA的纯度，并用琼脂糖凝胶电泳（1％）确认DNA。使用细胞色素c氧化酶亚基1（COI）基因对DNA提取物进行DNA条形码编码。用COI基因引物进行聚合酶链反应（PCR），使用100bp DNA阶梯通过琼脂糖凝胶电泳（2％）确认PCR产物的大小为652bp，并使用生物光度计（Eppendorf）分析DNA的含量。在260nm和280nm处测定DNA的纯度和浓度，PCR产物记录的纯度和浓度分别为1.58-1.63（DNA质量良好）和7.12（4.6-10.1）。对COI基因的PCR产物进行测序并提交给Genbank（NCBI），GenBank提供的Guentheri N.的登录号包括KP772692，KP772693，KP772694和KP772695。

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