...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of applied physiology >Exercise training initiated after the onset of diabetes preserves myocardial function: effects on expression of beta-adrenoceptors.
【24h】

Exercise training initiated after the onset of diabetes preserves myocardial function: effects on expression of beta-adrenoceptors.

机译：糖尿病发作后开始的运动训练可保留心肌功能：对β-肾上腺素受体表达的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study was undertaken to assess cardiac function and characterize beta-adrenoceptor subtypes in hearts of diabetic rats that underwent exercise training (ExT) after the onset of diabetes. Type 1 diabetes was induced in male Sprague-Dawley rats using streptozotocin. Four weeks after induction, rats were randomly divided into two groups. One group was exercised trained for 3 wk while the other group remained sedentary. At the end of the protocol, cardiac parameters were assessed using M-mode echocardiography. A Millar catheter was also used to assess left ventricular hemodynamics with and without isoproterenol stimulation. beta-Adrenoceptors were assessed using Western blots and [(3)H]dihydroalprenolol binding. After 7 wk of diabetes, heart rate decreased by 21%, fractional shortening by 20%, ejection fraction by 9%, and basal and isoproterenol-induced dP/dt by 35%. beta(1)- and beta(2)-adrenoceptor proteins were reduced by 60% and 40%, respectively, while beta(3)-adrenoceptor protein increased by 125%. Ventricular homogenates from diabetic rats bound 52% less [(3)H]dihydroalprenolol, consistent with reductions in beta(1)- and beta(2)-adrenoceptors. Three weeks of ExT initiated 4 wk after the onset of diabetes minimized cardiac function loss. ExT also blunted loss of beta(1)-adrenoceptor expression. Interestingly, ExT did not prevent diabetes-induced reduction in beta(2)-adrenoceptor or the increase of beta(3)-adrenoceptor expression. ExT also increased [(3)H]dihydroalprenolol binding, consistent with increased beta(1)-adrenoceptor expression. These findings demonstrate for the first time that ExT initiated after the onset of diabetes blunts primarily beta(1)-adrenoceptor expression loss, providing mechanistic insights for exercise-induced improvements in cardiac function.
机译：本研究旨在评估糖尿病发作后接受运动训练（ExT）的糖尿病大鼠心脏的心脏功能并鉴定其β-肾上腺素受体亚型。使用链脲佐菌素在雄性Sprague-Dawley大鼠中诱发1型糖尿病。诱导后四周，将大鼠随机分为两组。一组接受了3周的训练，而另一组则久坐不动。方案结束时，使用M型超声心动图评估心脏参数。 Millar导管还用于评估有或没有异丙肾上腺素刺激的左心室血流动力学。使用蛋白质印迹和[（3）H] dihydroalprenolol结合评估β-肾上腺素受体。糖尿病7周后，心率降低了21％，分数缩短了20％，射血分数降低了9％，基础和异丙肾上腺素引起的dP / dt降低了35％。 beta（1）-和beta（2）-肾上腺素受体蛋白分别减少60％和40％，而beta（3）-肾上腺素受体蛋白增加125％。来自糖尿病大鼠的心室匀浆结合[[3）H] dihydroalprenolol减少52％，与减少beta（1）-和beta（2）-肾上腺素能受体一致。糖尿病发作后4周开始三周的ExT，使心功能丧失降至最低。 ExT还钝化了beta（1）-肾上腺素受体表达的损失。有趣的是，ExT并未阻止糖尿病引起的β（2）-肾上腺素能受体减少或β（3）-肾上腺素能受体表达的增加。 ExT还增加了[（3）H] dihydroalprenolol绑定，与增加的beta（1）-肾上腺素受体表达一致。这些发现首次证明，在糖尿病发作后，ExT开始主要钝化β（1）-肾上腺素受体表达的丧失，从而为运动引起的心脏功能改善提供了机械学见解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号