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首页> 外文期刊>Journal of Applied Polymer Science >Preparation of porous polymeric structures for enzyme immobilization
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Preparation of porous polymeric structures for enzyme immobilization

机译：制备用于酶固定的多孔聚合物结构

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Porous polymethacrylic acid-co-triethylene glycol dimethacrylate (MAA-co-3G) and polyacrylic acid-co-triethylene glycol dimethacrylate (AA-co-3G) were prepared by four different polymerization techniques, namely, suspension, dispersion, seed, and microemulsion polymerization using an inert diluent (n-hexane and polystyrene). The morphology and porosity of the obtained polymers were examined by means of a scanning electron microscope. The surface areas of the obtained polymers were determined colorimetrically. The copolymers were modified by hydroximation and chlorination using hydroxyl amine and thionyl chloride, respectively. The effect of polymerization type, surface area, modification, and pH on the protease enzyme immobilization over poly(MAA-co-3G) and poly(AA-co-3G) was examined. The enzyme activity was measured by means of a spectrophotometer. The reactivity ratios of the two monomers MAA and 3G were determined by means of the elemental analysis method. (C) 2000 John Wiley & Sons, Inc. [References: 38]
机译：采用四种不同的聚合技术，分别是悬浮，分散，种子和微乳液，制备了多孔聚甲基丙烯酸-三乙二醇二甲基丙烯酸共聚物（MAA-co-3G）和聚丙烯酸-三乙二醇二甲基丙烯酸共聚物（AA-co-3G）。使用惰性稀释剂（正己烷和聚苯乙烯）进行聚合。用扫描电子显微镜检查所得聚合物的形态和孔隙率。用比色法测定所得聚合物的表面积。通过分别使用羟胺和亚硫酰氯的氢化和氯化来改性共聚物。考察了聚合类型，表面积，修饰度和pH对固定在聚（MAA-co-3G）和聚（AA-co-3G）上的蛋白酶的影响。酶活性通过分光光度计测量。两种单体MAA和3G的反应率通过元素分析法测定。 （C）2000 John Wiley＆Sons，Inc. [参考：38]

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