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首页> 外文期刊>Journal of Applied Genetics >LXR alpha gene expression, genetic variation and association analysis between novel SNPs and growth traits in Chinese native cattle.
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LXR alpha gene expression, genetic variation and association analysis between novel SNPs and growth traits in Chinese native cattle.

机译：中国本地牛的LXR alpha基因表达，遗传变异以及新型SNP与生长性状的关联分析。

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Liver X receptor alpha (LXR alpha ) has emerged as an important regulator of lipid and energy metabolism. In this study, to better understand the effects of LXR alpha gene on growth traits in cattle, the mRNA tissue expression patterns and the polymorphisms of some exons of LXR alpha were revealed. The expression profile of the bovine LXR alpha gene was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in 11 different Jiaxian cattle tissues and was found mainly expressed in spleen, liver, fat tissue, kidney, muscle, and lung. Meanwhile, it showed that four single nucleotide polymorphisms (SNPs), named g.1028 T > C, g.1514 T > C, g.2929G > A, and g.3493 T > C, were detected and 12 different haplotypes were constructed. Haplotype with CCGT was dominant with frequency of 40.8%. There was a strong link between g.1028 T > C and g.1514 T > C (r2=0.374). Association analysis of SNPs with growth- and body-related traits was carried out in 445 Chinese native cattle. The results displayed that the heterozygous genotypes of g.1028 T > C and g.1514 T > C showed a molecular heterosis on four performance traits related to body size: height at withers, body length, hipbone width, and hip width (P<0.05). The multiple effects of four sites showed that the height at withers, body length, hipbone width, and hip width of individuals of TC-TC-GG-TT combined genotypes were significantly higher than other genotypes (P<0.05). The effects of the four loci genotype combination on conformation traits were consistent with the effects of g.1028 T > C and g.1514 T > C loci. The SNPs of g.1028 T > C and g.1514 T > C of the bovine LXR alpha gene could be potential genetic markers for growth traits in cattle. These results suggest that LXR alpha gene is expressed in many tissues and may provide primary molecular information for further studies on body size traits in Chinese indigenous cattle.
机译：肝脏X受体α（LXR alpha）已成为脂质和能量代谢的重要调节剂。在这项研究中，为了更好地了解LXR alpha基因对牛生长特性的影响，揭示了LXR alpha的一些外显子的mRNA组织表达模式和多态性。通过定量实时聚合酶链反应（qRT-PCR）检测了牛LXR alpha基因在11种Jia县牛组织中的表达谱，发现其主要表达于脾脏，肝脏，脂肪组织，肾脏，肌肉和肺脏。同时，它表明检测到四个单核苷酸多态性（SNP），分别为g.1028 T> C，g.1514 T> C，g.2929G> A和g.3493 T> C，并构建了12个不同的单倍型。 CCGT单倍型占优势，频率为40.8％。 g.1028 T> C和g.1514 T> C之间有很强的联系（r 2 = 0.374）。在445名中国本地牛中进行了SNP与生长和身体相关性状的关联分析。结果显示，g.1028 T> C和g.1514 T> C的杂合基因型在与体型有关的四个性能特征上表现出分子杂种优势：肩高，体长，髋骨宽度和髋部宽度（P < 0.05）。四个位点的多重效应表明，TC-TC-GG-TT联合基因型个体的肩ers高，体长，髋骨宽度和臀部宽度显着高于其他基因型（P <0.05）。四个基因座基因型组合对构象性状的影响与g.1028 T> C和g.1514 T> C基因座的影响一致。牛LXR alpha基因的g.1028 T> C和g.1514 T> C的SNPs可能是牛生长性状的潜在遗传标记。这些结果表明，LXRα基因在许多组织中表达，并可能为进一步研究中国本土牛体型特征提供主要的分子信息。

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