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首页> 外文期刊>Journal of Applied Polymer Science >A New Metal Chelate Sorbent for Glucose Oxidase Cu(II)-and Co(II) -Chelated Poly(N-vinyimidazole) Gels
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A New Metal Chelate Sorbent for Glucose Oxidase Cu(II)-and Co(II) -Chelated Poly(N-vinyimidazole) Gels

机译：葡萄糖氧化酶Cu（II）-和Co（II）-螯合的聚（N-乙烯基咪唑）凝胶的新型金属螯合吸附剂

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Poly(N-vinylimidazole)(PVIm) gels were prepared by irradiating a binary mixture of N-vinylimidazole(VIm) -water in a ~(60) Co-#gamma# source having 4.5kG/h dose rate.In the glucose oxidase(GOx) adsorption studies,affinity gels with a swelling ratio of 1100%for PRVm and 40 and 55% for Cu(II)-and Co(II)-chelated PVIm gels,respectively,at pH6.5 in phosphate buffer were used,FTIR spectra were taken for PVIm and Cu(II)-and Co(II)-chelated PVIm, and glucose oxidase adsorption on these gels .,to characterize the nature of the interactions in each species .The results show that PVIm-glucose oxidase interaction is mainly electrostatic and metal ion-chelated PVIm gel-glucose oxidase interaction is of coordinate covalent nature. Cu(II)-and Co(II) ions were chelated within the gels via groups on the imidazole ring of the gel.Different amounts f Cu(II)-and Co(II) ions [maximum 3.64 mmol/g dry gel for Cu(II)and 1.72 mmol/g dry gel for Co(II)]ions at pH7.0 Gox adsorption on these gels from aqueous solutions containing different amounts of GOx at different pH was investigated in batch reactors.Gox adsorption capacity was further increased when Cu(II) and Co(II) ions were attached[up to 0.53 g Gox/g dry Co(II) -chelated PVIm gels ].More than 90% of the adsorbed Gox was desorbed in 5 h in desorption medium containing 1.0 M DSCN at pH7.0 for plain gel and 0.05M EDTA at pH 4.9 for metal-chelated gel.Nonpecific glucose oxidase adsorption on/in the metal ion-chelated PVIm gel was investigated using 0.02M of phosphate buffer solution The nonspecific Gox adsorption was determined to be about 18% for PVIm and 8% for the metal ion-chelated PVIm gels .The ionic strength effect was investigated both on PVIm and on the metalion -chelated PVIm gels for the glucose oxidase adsorption .It was found that ionic strength was more effective on the PVIm gel because of the electrostatic interaction between protonated gel and the deprotonated glucose oxidase side chain.
机译：聚（N-乙烯基咪唑）（PVIm）凝胶是通过在剂量率为4.5kG / h的〜（60）Co-γ-源中辐照N-乙烯基咪唑（VIm）-水的二元混合物制得的。 （GOx）吸附研究，使用亲和性凝胶，分别在pH6.5和磷酸盐缓冲液中，溶胀率分别为PRVm的1100％和Cu（II）和Co（II）螯合的PVIm凝胶的40和55％。用FTIR光谱对PVIm和Cu（II）和Co（II）螯合的PVIm进行了吸附，并在这些凝胶上吸附了葡萄糖氧化酶，以表征每种物种之间相互作用的性质。结果表明，PVIm-葡萄糖氧化酶相互作用主要是静电与金属离子螯合的PVIm凝胶-葡萄糖氧化酶的相互作用具有配位共价性。 Cu（II）和Co（II）离子通过凝胶上的咪唑环上的基团螯合在凝胶中。不同数量的f Cu（II）和Co（II）离子[对于Cu的最大干凝胶为3.64 mmol / g （II）和1.72 mmol / g Co（II）]离子的干凝胶，在间歇反应器中研究了这些凝胶在不同pH值下从含有不同量GOx的水溶液中吸附到这些凝胶上的Gox。当将Cu（II）和Co（II）离子附着[高达0.53 g Gox / g干燥的Co（II）螯合的PVIm凝胶]。超过90％的吸附Gox在含有1.0 M的解吸介质中解吸5小时。普通凝胶的pH7.0为DSCN，金属螯合的凝胶为pH4.9的EDTA为0.05M EDTA。使用0.02M磷酸盐缓冲溶液研究了非特定葡萄糖氧化酶在金属离子螯合的PVIm凝胶上/中的非特异性吸附对于PVIm约为18％，对于金属离子螯合的PVIm凝胶约为8％。研究了离子强度对PVIm和金属离子的影响螯合的PVIm凝胶用于葡萄糖氧化酶的吸附。发现离子强度对PVIm凝胶更有效，因为质子化的凝胶与去质子化的葡萄糖氧化酶侧链之间存在静电相互作用。

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