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UV-Cured Natural Polymer-Based Membrane for Biosensor Application

机译:用于生物传感器应用的紫外线固化的天然聚合物基膜

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摘要

Chitosan, a natural product, is inherently biodegradable, biocompatible, and nontoxic. These properties make chitosan iedal for inclusion in matrics designed for use in enzyme immobilization for clinical analysis. This study demonsrates the feasibility of using chitosan in electrochemical biosensor fabrication. The enzyme slfite oxidase (SOX) was covalently immobilized onto the matrix of chitosan-poly(hydroxyethyl methacrylate) (chitosan-pHEMA), a natural/synthetic polymer hybrid obtainable via UV curing, p-Benzoquinone, which served as an electron transfer mediator, was coupled onto the photo-induced polymerization reaction. The biological activity of the immobilized SOX and the electroactivity of the coupled p-benzoquinone were investigated.
机译:壳聚糖是一种天然产品,具有固有的生物降解性,生物相容性和无毒性。这些特性使壳聚糖能够被包含在设计用于酶固定化用于临床分析的基质中。这项研究表明了在化学生物传感器制造中使用壳聚糖的可行性。将亚硫酸盐氧化酶(SOX)共价固定在壳聚糖-聚(甲基丙烯酸羟乙酯)(壳聚糖-pHEMA)的基质上,壳聚糖-聚甲基丙烯酸羟乙酯是一种通过UV固化而获得的天然/合成聚合物杂化物,对-苯醌用作电子转移介体,被耦合到光致聚合反应上。研究了固定化SOX的生物活性和偶联的对苯醌的电活性。

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