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首页> 外文期刊>Journal of Applied Polymer Science >Metal-chelated polyamide hollow fibers for human serum albumin separation
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Metal-chelated polyamide hollow fibers for human serum albumin separation

机译：金属螯合聚酰胺中空纤维用于人血清白蛋白分离

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We modified microporous polyamide hollow fibers by acid hydrolysis to amplify the reactive groups and subsequent binding of Cibacron Blue F3GA. Then, we loaded the Cibacron Blue F3GA-attached hollow fibers with different metal ions (CU2+, Ni2+, and Co2+) to form the metal chelates. We characterized the hollow fibers by scanning electron microscopy. The effect of pH and initial concentration of human serum albumin (HSA) on the adsorption of HSA to the metal-chelated hollow fibers were examined in a batch system. Dye- and metal-chelated hollow fibers had a higher HSA adsorption capacity and showed less nonspecific protein adsorption. The nonspecific adsorption of HSA onto the polyamide hollow fibers was 6.0 mg/g. Cibacron Blue F3GA immobilization onto the hollow fibers increased HSA adsorption up to 147 mg/g. Metal-chelated hollow fibers showed further increases in the adsorption capacity. The maximum adsorption capacities of Co2+-, Cu2+-, and Ni2+ -chelated hollow fibers were 195, 226, and 289 mg/g, respectively. The recognition range of metal ions for HSA from human serum followed the order: Ni(II) > Cu(II) > Co(II). A higher HSA adsorption was observed from human serum (324 mg/g). A significant amount of the adsorbed HSA (up to 99%) was eluted for I h in the elution medium containing 1.0M sodium thiocyanide (NaSCN) at pH 8.0 and 25 mM ethylenediaminetetraacetic acid at pH 4.9. Repeated adsorption-desorption processes showed that these metal-chelated polyamide hollow fibers were suitable for HSA adsorption. (C) 2002 Wiley Periodicals, Inc. [References: 42]
机译：我们通过酸水解修饰了微孔聚酰胺中空纤维，以放大反应基团并随后结合Cibacron Blue F3GA。然后，我们用不同的金属离子（CU2 +，Ni2 +和Co2 +）加载连接Cibacron Blue F3GA的中空纤维，以形成金属螯合物。我们通过扫描电子显微镜表征了中空纤维。 pH和人血清白蛋白（HSA）初始浓度对HSA吸附在金属螯合中空纤维上的影响已通过分批系统进行了研究。染料和金属螯合的中空纤维具有较高的HSA吸附能力，并显示较少的非特异性蛋白质吸附。 HSA在聚酰胺中空纤维上的非特异性吸附为6.0 mg / g。将Cibacron Blue F3GA固定在中空纤维上可将HSA吸附提高到147 mg / g。金属螯合中空纤维的吸附能力进一步提高。 Co2 +-，Cu2 +-和Ni2 +螯合的中空纤维的最大吸附容量分别为195、226和289 mg / g。人类血清中HSA的金属离子的识别范围依次为：Ni（II）> Cu（II）> Co（II）。从人血清中观察到更高的HSA吸附（324 mg / g）。在含有1.0M硫氰化钠（NaSCN）（pH 8.0）和25 mM乙二胺四乙酸（pH 4.9）的洗脱介质中，将大量吸附的HSA（最高99％）洗脱1小时。重复的吸附-解吸过程表明，这些金属螯合的聚酰胺中空纤维适用于HSA吸附。 （C）2002 Wiley Periodicals，Inc. [参考：42]

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