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首页> 外文期刊>Journal of Applied Polymer Science >Human serum albumin (HSA) adsorption with chitosan microspheres
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Human serum albumin (HSA) adsorption with chitosan microspheres

机译：壳聚糖微球吸附人血清白蛋白（HSA）

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摘要

In this study, chitosan microspheres were prepared and characterized for adsorption of human serum albumin (HSA) as affinity sorbent. The chitosan microspheres were obtained with a "suspension crosslinking technique" in the size range of 30-700 mum by using a crosslinker, i.e., glutaraldehyde. The chitosan microspheres used in HSA adsorption studies were having the average size of 170 +/- 81 mum. Adsorption medium pH and the initial HSA concentration in the adsorption medium were changed as 4.0-7.0 and 0.5-2.0 mg HSA/mL, respectively, to investigate the HSA adsorption capacity of chitosan microspheres. Maximum HSA adsorption (i.e., 11.35 mg HSA/g chitosan microspheres) was obtained at pH 5.0 and 1.5 mg HSA/mL of the initial HSA concentration in the adsorption medium was obtained as the saturation value for HSA adsorption. A very common dye ligand, i.e., Cibacron Blue F3GA was attached to the chitosan microspheres to increase the HSA adsorption capacity. Actually, the HSA adsorption capacity was increased up to 15.35 mg HSA/g chitosan microspheres in the case of Cibacron Blue F3GA attached to chitosan microspheres used. (C) 2002 Wiley Periodicals, Inc. [References: 24]
机译：在这项研究中，制备了壳聚糖微球并表征了其吸附人血清白蛋白（HSA）的亲和力。使用“悬浮交联技术”，通过使用交联剂，即戊二醛，在30-700μm的尺寸范围内获得壳聚糖微球。用于HSA吸附研究的壳聚糖微球的平均大小为170 +/- 81微米。吸附介质的pH值和吸附介质中的HSA初始浓度分别更改为4.0-7.0和0.5-2.0 mg HSA / mL，以研究壳聚糖微球的HSA吸附能力。在pH 5.0下获得最大的HSA吸附（即11.35mg HSA / g壳聚糖微球），并且获得1.5mg HSA / mL的吸附介质中的初始HSA浓度作为HSA吸附的饱和值。将非常常见的染料配体即Cibacron Blue F3GA连接至壳聚糖微球以增加HSA吸附能力。实际上，在将Cibacron Blue F3GA附着在所使用的壳聚糖微球上的情况下，HSA吸附容量增加到高达15.35 mg HSA / g壳聚糖微球。 （C）2002 Wiley Periodicals，Inc. [参考：24]

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