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首页> 外文期刊>Journal of Applied Polymer Science >CHITOSAN/CALCIUM-ALGINATE BEADS FOR ORAL DELIVERY OF INSULIN
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CHITOSAN/CALCIUM-ALGINATE BEADS FOR ORAL DELIVERY OF INSULIN

机译：壳聚糖/海藻酸钙珠口服治疗胰岛素

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摘要

A mild chitosan/calcium alginate microencapsulation process, as applied to encapsulation of biological macromolecules such as albumin and insulin, was investigated. The microcapsules were derived by adding dropwise a protein-containing sodium alginate mixture into a chitosan-CaCl2 system. The beads containing a high concentration of entrapped bovine serum albumin (BSA) as more than 70% of the initial concentration were achieved via varying chitosan coat. It was observed that approximately 70% of the content is being released into Tris-HCl buffer, pH 7.4 within 24 h and no significant release of BSA was observed during treatment with 0.1M HCl pH 1.2 for 4 h. But the acid-treated beads had released almost all the entrapped protein into Tris-HCl pH 7.4 media within 24 h. Instead of BSA, the insulin preload was found to be very low in the chitosan/calcium alginate system; the release characteristics were similar to that of BSA. From scanning electron microscopic studies, it appears that the chitosan modifies the alginate microspheres and subsequently the protein loading. The results indicate the possibility of modifying the formulation in order to obtain the desired controlled release of bioactive peptides (insulin), for a convenient gastrointestinal tract delivery system. (C) 1996 John Wiley & Sons, Inc. [References: 24]
机译：研究了一种温和的壳聚糖/藻酸钙微囊化工艺，该工艺应用于生物大分子（如白蛋白和胰岛素）的囊封。通过将含蛋白质的海藻酸钠混合物滴加到壳聚糖-CaCl2系统中来获得微胶囊。通过改变脱乙酰壳多糖涂层获得了含有高浓度的被捕获的牛血清白蛋白（BSA）（超过初始浓度的70％）的珠子。观察到约有70％的内含物在24小时内释放到pH 7.4的Tris-HCl缓冲液中，在用0.1M HCl pH 1.2处理4小时的过程中未观察到BSA的明显释放。但是经过酸处理的微珠在24小时内将几乎所有截留的蛋白质释放到了Tris-HCl pH 7.4介质中。发现壳聚糖/海藻酸钙系统中的胰岛素预载量很低，而不是BSA。释放特性类似于BSA。从扫描电子显微镜研究看来，壳聚糖修饰了藻酸盐微球并随后修饰了蛋白质负载量。结果表明为了方便的胃肠道递送系统，为了获得所需的生物活性肽（胰岛素）的受控释放，可以改变制剂的可能性。 （C）1996 John Wiley＆Sons，Inc. [参考：24]

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