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首页> 外文期刊>Journal of Applied Polymer Science >NOVEL DIAZONIUM-FUNCTIONALIZED SUPPORT FOR IMMOBILIZATION EXPERIMENTS
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NOVEL DIAZONIUM-FUNCTIONALIZED SUPPORT FOR IMMOBILIZATION EXPERIMENTS

机译：新型重氮功能支持的免疫实验

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摘要

A hydrophilic, water-insoluble polymer was prepared, starting from commercial poly(vinyl alcohol) that was crosslinked and functionalized by means of glutaraldehyde and 4-nitrobenzaldehyde. The resulting beads were then reduced and subsequently diazotized, and finally contained diazonium moieties capable of covalently coupling with electron-rich aromatic systems such as histidine and/or tyrosine residues of proteins. The described resin is therefore well suitable for protein immobilization whenever lysine residues (those involved in covalent coupling with several popular immobilization procedures) are not available and/or cannot be used unless the biological activity of the protein is destroyed. (C) 1997 John Wiley & Sons, Inc. [References: 18]
机译：从商业的聚乙烯醇开始制备亲水性，水不溶性聚合物，该聚乙烯醇通过戊二醛和4-硝基苯甲醛交联并官能化。然后将所得的珠还原并随后重氮化，并且最后包含能够与富电子芳族系统例如组氨酸和/或蛋白质的酪氨酸残基共价偶联的重氮部分。因此，只要赖氨酸残基（与几种流行的固定方法共价偶联的赖氨酸残基）不可用和/或无法使用，除非所述蛋白质的生物学活性被破坏，否则所述树脂非常适合于蛋白质固定。 （C）1997 John Wiley＆Sons，Inc. [参考：18]

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