首页> 外文期刊>Journal of Applied Animal Research >Simple multiplex PCR for rapid diagnosis of sex of ducks and duck embryos.
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Simple multiplex PCR for rapid diagnosis of sex of ducks and duck embryos.

机译:简单的多重PCR可快速诊断鸭子和鸭子胚胎的性别。

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摘要

A simple and rapid multiplex polymerase chain reaction (PCR) was developed for the rapid sex diagnosis of ducks and duck embryos. A sex chromosome-specific DNA sequence was selected and primers were designed to amplify a 335-bp fragment from femaled, while a 16S ribosomal sequence was selected to design primers to amplify a 468-bp PCR products in males and females as an internal control. Nucleotide sequences of sex chromosome-specific DNA fragments of Khaki Campbell and Indian Runner duck breeds were identical both in size and sequences. This protocol is successful in precisely identifying the gender of ducklings belonging to both Indian Runner and Khaki Campbell breeds of ducks and duck embryos.
机译:开发了一种简单快速的多重聚合酶链反应(PCR),用于快速诊断鸭子和鸭子胚胎的性别。选择了性染色体特异性的DNA序列,并设计了引物以扩增雌性的335 bp片段,同时选择了16S核糖体序列以设计引物以扩增雄性和雌性中的468 bp PCR产物作为内部对照。卡其·坎贝尔和印度流浪鸭品种的性染色体特异性DNA片段的核苷酸序列在大小和序列上都相同。该协议可以成功地准确地识别属于印度跑者和卡其坎伯鸭种以及鸭胚胎的小鸭的性别。

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