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首页> 外文期刊>Journal of Applied Polymer Science >Development of polysulfone membranes for bacteria immobilization to remove phenol
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Development of polysulfone membranes for bacteria immobilization to remove phenol

机译：用于固定细菌去除苯酚的聚砜膜的开发

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We have investigated the feasibility of developing polysulfone (PS) membranes to partially immobilize Pseudomonas and to evaluate the inhibitory effect of phenol on immobilized Pseudomonas by monitoring their growths in partially immobilized cell and free-suspension systems. The polysulfone membranes used in this study were wet spun from 20 wt % of PS in 1-methyl-2-pyrrolidone (NMP) solvent using water as the bore fluid as well as the external coagulant. Scanning electron microscopy (SEM) characterization of the newly developed PS hollow fibers suggests that fiber cross-section consists of multilayer microporous structures useful for cell immobilization. Experiments were conducted using Pseudomonas bacteria to remove phenol with initial phenol concentrations of 300 mg/L and 1000 mg/L. In a free suspension (no membrane) system, it was observed that the bacteria were able to grow optimally at 300 mg/L of phenol and degraded phenol almost completely in about 26 h. However, neither cell growth nor phenol degradation occurred when initial concentration of phenol was increased to 1000 mg/L. In a cell-immobilized membrane system, the cell growth and phenol concentration profile in the medium were very similar to those obtained in a free-suspension culture if phenol concentration was 300 mg/L. However, when the initial phenol concentration was increased to 1000 mg/L, data obtained in a cell-immobilized membrane system was discernibly different from that obtained in the suspension culture. In the former case, phenol concentration decreased in the beginning of the test, indicating that the carbon source has been consumed and immobilized cells within the membrane had begun to multiply. As soon as the phenol concentration decreased to about 600 mg/L (at which concentration, substrate inhibition was not as severe as 1000 mg/L), partial immobilization occurred when some cells diffused out of the membrane into the medium and optical density became measurable in the medium. It was found that cell growth continued for the next 28 h, reaching a maximum optical density in the medium of 0.610 absorbance units, and phenol was also completely degraded. (C) 1998 John Wiley & Sons, Inc. [References: 28]
机译：我们已经研究了开发聚砜（PS）膜部分固定化假单胞菌的可行性，并通过监测其在部分固定化细胞和自由悬浮系统中的生长来评估苯酚对固定化假单胞菌的抑制作用。本研究中使用的聚砜膜是从20 wt％的PS在1-甲基-2-吡咯烷酮（NMP）溶剂中湿纺而成的，使用水作为钻孔液以及外部凝结剂。新开发的PS中空纤维的扫描电子显微镜（SEM）表征表明，纤维横截面由可用于细胞固定的多层微孔结构组成。使用假单胞菌细菌进行实验以去除初始苯酚浓度分别为300 mg / L和1000 mg / L的苯酚。在自由悬浮液（无膜）系统中，观察到细菌能够以约300 mg / L的苯酚最佳生长，并在约26小时内几乎完全降解苯酚。但是，当苯酚的初始浓度增加到1000 mg / L时，细胞生长和苯酚降解都不会发生。在细胞固定膜系统中，如果苯酚浓度为300 mg / L，则培养基中的细胞生长和苯酚浓度曲线与在自由悬浮培养中获得的非常相似。但是，当初始苯酚浓度增加到1000 mg / L时，在细胞固定膜系统中获得的数据与悬浮培养中获得的数据明显不同。在前一种情况下，苯酚浓度在测试开始时就降低了，这表明碳源已经被消耗掉了，膜中固定的细胞开始繁殖。苯酚浓度降至约600 mg / L（在该浓度下，底物抑制作用不如1000 mg / L严重），当一些细胞从膜中扩散到培养基中并可以测量光密度时，发生部分固定化在中等。发现细胞生长持续接​​下来的28小时，在0.610吸光度单位的培养基中达到最大光密度，苯酚也被完全降解。 （C）1998 John Wiley＆Sons，Inc. [参考：28]

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