首页> 外文期刊>Journal of Applied Polymer Science >Covalent immobilization of bovine serum albumin onto (maleic anhydride-alt-methyl vinyl ether) copolymers
【24h】

Covalent immobilization of bovine serum albumin onto (maleic anhydride-alt-methyl vinyl ether) copolymers

机译:牛血清白蛋白共价固定在(马来酸酐-alt-甲基乙烯基醚)共聚物上

获取原文
获取原文并翻译 | 示例
           

摘要

The covalent immobilization of bovine serum albumin (BSA) onto maleic anhydride-alt-methyl vinyl ether copolymers (MAMVE) was successfully achieved under aqueous conditions. The grafting reaction was shown to be controlled by attractive electrostatic interactions and so took place at a low salt concentration. Under these conditions, the covalent binding reaction was quite efficient, reproducible, and complete within 20 min. The maximum loading capacity of the polymer was of 20 BSA molecules per polymer chain. This preliminary study demonstrated that the immobilization of proteins in an aqueous medium could be an efficient process, despite the existing hydrolysis of the functional polymer by water molecules.
机译:在水性条件下成功地将牛血清白蛋白(BSA)共价固定在马来酸酐-alt-甲基乙烯基醚共聚物(MAMVE)上。接枝反应显示出受吸引的静电相互作用控制,因此在低盐浓度下发生。在这些条件下,共价结合反应非常有效,可重现并在20分钟内完成。聚合物的最大负载量为每条聚合物链20个BSA分子。这项初步研究表明,尽管功能分子已被水分子水解,但将蛋白质固定在水性介质中可能是一个有效的过程。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号