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首页> 外文期刊>Journal of Applied Polymer Science >Reactivity of Temperature-Sensitive, Protein-Conjugating Polymers Prepared by a Photopolymerization Process
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Reactivity of Temperature-Sensitive, Protein-Conjugating Polymers Prepared by a Photopolymerization Process

机译：通过光聚合工艺制备的温度敏感蛋白偶联聚合物的反应性

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摘要

This study was carried out to characterize the reactivity of temperature sensitive, protein-conjugating polymers prepared by a photopolymerization process. Polymers were based on N-isopropylacrylamide (NiPAM) and N-acryloxysuccinimide (NASI). A photoninitiator, 2,2-dimethoxy-2-phenyl-acetophenone, and monomers at desired ratios were polymerized in a glass flask using an UV source. Polymers were characterized for composition, molecular weight (MW), cloud point temperature (CPT), hydrolysis and aminolysis rates (using ethanolamine as a model compound), and protein conjugation. The monomer feed ratio was found to effectively control the composition of the synthesized polymers. The polymer MWs were between 10 and 20 kD, depending on the polymerization solvent. The CPT of NiPAM/NASI polymers did not depend on SASI content (<=5.6%), nor did the hydrolysis and aminolyis rates. Compared to NASI monomer, the polymerized NASI exhibited a 6- and 120-fold slower rates of hydrolysis and aminolysis, respectively. Although hydrolysis and aminolysis rates were hgiher at higher pHs, the relative aminolysis: hydrolysis rate was highest at a pH of 7.4, which also gave the most effective protein conjugation. We conclude that characterizing the polymer reactivity is useful for predicting the optimal conditions for protein conjugation and may faciltate the design of polymers with improved protein conjugation kinetics.
机译：进行该研究以表征通过光聚合过程制备的温度敏感性蛋白偶联聚合物的反应性。聚合物基于N-异丙基丙烯酰胺（NiPAM）和N-丙烯酰氧基琥珀酰亚胺（NASI）。使用紫外线源，在玻璃烧瓶中将所需比例的光引发剂，2，2-二甲氧基-2-苯基-苯乙酮和单体聚合。表征聚合物的组成，分子量（MW），浊点温度（CPT），水解和氨解速率（使用乙醇胺作为模型化合物）和蛋白质结合。发现单体进料比可有效控制合成聚合物的组成。取决于聚合溶剂，聚合物的MW为10至20kD。 NiPAM / NASI聚合物的CPT不依赖于SASI含量（<= 5.6％），也不依赖于水解和氨基裂解速率。与NASI单体相比，聚合后的NASI的水解和氨解速度分别慢6到120倍。尽管在较高的pH值下水解和氨解速率较高，但是相对的氨解：在7.4的pH值下水解速率最高，这也使蛋白质结合最有效。我们得出结论，表征聚合物的反应性可用于预测蛋白质缀合的最佳条件，并可能有助于设计具有改善的蛋白质缀合动力学的聚合物。

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