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首页> 外文期刊>Journal of applied toxicology >Cadmium accumulation and binding characteristics in intact Sertoli/germ cell units, and associated effects on stage-specific functions in vitro: insights from a shark testis model.
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Cadmium accumulation and binding characteristics in intact Sertoli/germ cell units, and associated effects on stage-specific functions in vitro: insights from a shark testis model.

机译：完整的Sertoli /生殖细胞单元中的镉积累和结合特征，以及对体外特定阶段功能的相关影响：来自鲨鱼睾丸模型的见解。

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The increased human use of cadmium (Cd) and its increased occurrence in the environment is of concern. The testis is sensitive to Cd because of the steroid-mediated regulation of spermatogenesis, high levels of DNA synthesis and gene transcription, all of which varies in a stage-related manner. Validated techniques (acridine orange vital staining to detect apoptosis and dextran-rhodamine exclusion to assess blood-testis barrier function) were recently developed and the shark testis was proposed as an alternative model for assessing stage-specific functions in living testicular tissue and to study toxicant actions on spermatogenesis. The present paper shows that 109Cd accumulation and binding in vitro was stage-dependent (premeiotic, PrM > meiotic, M > postmeiotic, PoM), rapid and persisted in spermatocysts (intact germ cell/Sertoli cell units) 49 h after washout. In competitive binding analyses of all three spermatocyst stages, Hg, but not Zn, could replace bound 109Cd, suggesting that Cd binding was specific. These findings were associated with a biphasic apoptotic response in the PrM spermatocysts, which was maximal at 10 microm CdCl2 and 1 microm CdCl2 after 2 and 4 days in culture, respectively. Although Cd uptake in PoM cysts was more than 2-fold less than uptake in PrM cysts, the percentage dextran-rhodamine permeant PoM cysts was approximately 8-fold greater than in controls in the presence of both 10 microm CdCl2 and 30 microm CdCl2 after 4 days culture, indicating that blood-testis barrier function in PoM spermatocysts was compromised. These findings demonstrate that this model has utility for use in screening assays of environmental toxicants.
机译：人们越来越关注镉（Cd）的使用及其在环境中的增加。由于类固醇介导的精子发生调节，高水平的DNA合成和基因转录，睾丸对Cd敏感，所有这些都以阶段相关的方式变化。最近开发了经过验证的技术（ac啶橙活力染色以检测细胞凋亡和右旋糖精-若丹明排斥以评估血液-睾丸屏障功能），并提出了鲨鱼睾丸作为评估活体睾丸组织中阶段特异性功能和研究有毒物质的替代模型。对生精的作用。本论文表明，109Cd在体外的积累和结合具有阶段依赖性（减数分裂前，PrM>减数分裂，M>减数分裂后的PoM），并且在冲洗后49 h迅速并持续存在于精囊（完整生殖细胞/ Sertoli细胞单位）中。在所有三个精子囊阶段的竞争性结合分析中，Hg而不是Zn可以代替结合的109Cd，这表明Cd结合具有特异性。这些发现与PrM精囊中的双相凋亡反应有关，在培养2天和4天后最大分别在10微米CdCl2和1微米CdCl2处。尽管PoM囊肿中Cd的吸收量比PrM囊肿中的Cd吸收量少2倍以上，但在10μmCdCl2和30 microd CdCl2的存在下，右旋糖精-罗丹明渗透PoM囊肿的百分比大约比对照组高8倍。天培养表明PoM精囊的血液-睾丸屏障功能受到损害。这些发现表明，该模型可用于筛选环境有毒物质。

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