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首页> 外文期刊>Journal of applied toxicology >Blocking of intracellular ROS production by phytoglycoprotein (30 kDa) causes anti-proliferation in bisphenol A-stimulated Chang liver cells.
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Blocking of intracellular ROS production by phytoglycoprotein (30 kDa) causes anti-proliferation in bisphenol A-stimulated Chang liver cells.

机译：植物糖蛋白（30 kDa）阻止细胞内ROS的产生会引起双酚A刺激的Chang肝细胞中的抗增殖。

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摘要

Dioscorea batatas Decne (DBD) is traditionally used to heal inflammatory disease as a folk medicine. It was reported that a glycoprotein (DBD glycoprotein) with a molecular weight of 30 kDa was isolated from DBD and consists of carbohydrate (83.75%) and protein (16.25%) moieties. The previous results showed that it has a strong scavenging activity against hydroxyl radicals without any pro-oxidant activity in the cell-free system. The purpose of the present study was to show whether or not the DBD glycoprotein inhibits cell proliferation-related signal transduction stimulated by bisphenol A (BPA, an environmental hormone) in Chang liver cells. The results in this study indicated that DBD glycoprotein (200 microg ml(-1)) has suppressive effects on abnormal cell viability, production of intracellular reactive oxygen species (ROS) and nitric oxide (NO) in BPA (50 microM)-induced Chang liver cells by blocking the phosphorylation of mitogen-activated protein kinase (MAPK) and activating protein-1 (AP-1) activity. In addition, DBD glycoprotein (200 microg ml(-1)) normalized the activity of catalase (CAT) and glutathione peroxidase (GPx). Consequently, DBD glycoprotein inhibits the expression of proliferating cell nuclear antigen (PCNA, cell proliferation maker) stimulated by BPA. Therefore, it is speculated that DBD glycoprotein protects against carcinogenic events caused by BPA in Chang liver cells.
机译：传统上，薯（Dioscorea batatas Decne，DBD）可作为一种民间药来治愈炎症。据报道，从DBD中分离出分子量为30kDa的糖蛋白（DBD糖蛋白），其由碳水化合物（83.75％）和蛋白质（16.25％）部分组成。先前的结果表明，它对羟基自由基具有很强的清除活性，而在无细胞系统中却没有任何促氧化剂的活性。本研究的目的是显示DBD糖蛋白是否抑制由双酚A（环境激素）双酚A刺激的肝细胞增殖相关的信号转导。这项研究的结果表明，DBD糖蛋白（200 microg ml（-1））对BPA（50 microM）诱导的Chang的异常细胞活力，细胞内活性氧（ROS）和一氧化氮（NO）的产生具有抑制作用。肝细胞通过阻断丝裂原激活的蛋白激酶（MAPK）的磷酸化和激活蛋白1（AP-1）的活性。此外，DBD糖蛋白（200 microg ml（-1））使过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性正常化。因此，DBD糖蛋白抑制了BPA刺激的增殖细胞核抗原（PCNA，细胞增殖制造者）的表达。因此，推测DBD糖蛋白可保护肠肝细胞中BPA引起的致癌事件。

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