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首页> 外文期刊>Journal of applied toxicology >Benomyl induction of brain aromatase and toxic effects in the zebrafish embryo.
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Benomyl induction of brain aromatase and toxic effects in the zebrafish embryo.

机译：苯菌灵诱导脑芳香化酶和斑马鱼胚胎中的毒性作用。

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Benomyl is a benzimidazole fungicide that has been widely used on a variety of food crops and ornamental plants. It is known to cause adverse effects on reproductive systems, including decreased testicular and epididymal weights and reduced epididymal sperm counts and fertility. The brain aromatase gene is up-regulated by estrogens and estrogen mimics and considered a target gene to screen estrogen mimics. This study was designed to test the estrogenic potential and toxic effects of benomyl in the zebrafish system, and validated this system as a model that may correspond to the effect of benomyl in rodents. Concentrations of 20 x 10(-6), 40 x 10(-6) and 80 x 10(-6) M of benomyl-treated embryos showed decreased survival, hatching and heart rates, and increased incidence of malformations, such as pericardial edema, spinal lordosis, elongated heart, head edema, eye lens protrusion and caudal fin disappearance. Benomyl induced enhanced green fluorescent protein (EGFP) expression in the mediobasal hypothalamus (MBH) in transient zebrafish embryos with a brain aromatase-based reporter gene. In this study, we determined that benomyl has estrogenic potential based on zebrafish brain aromatase gene induction, and that benomyl is toxic at 20 x 10(-6) M concentration and higher. These results demonstrate the usefulness of zebrafish embryos as an in vivo system to examine the estrogenic and developmental toxic potential of unknown compounds.
机译：苯菌灵是一种苯并咪唑类杀菌剂，已广泛用于各种粮食作物和观赏植物。已知会对生殖系统造成不利影响，包括睾丸和附睾重量减少以及附睾精子数量和生育力降低。脑芳香酶基因被雌激素和雌激素模拟物上调，被认为是筛选雌激素模拟物的靶基因。这项研究旨在测试苯菌灵在斑马鱼系统中的雌激素潜力和毒性作用，并验证了该系统作为一种模型可能与苯菌灵在啮齿类动物中的作用相对应。浓度20 x 10（-6），40 x 10（-6）和80 x 10（-6）M的苯菌灵处理的胚胎显示出存活率，孵化率和心率降低，畸形发生率（如心包水肿）增加，脊柱前凸，心脏延长，头水肿，眼晶状体突出和尾鳍消失。苯菌灵诱导的斑马鱼胚胎中脑下丘脑（MBH）中的绿色荧光蛋白（EGFP）表达增强，具有基于脑芳香酶的报告基因。在这项研究中，我们确定了基于苯并fish鱼脑芳香酶基因诱导的苯菌灵具有雌激素潜力，并且苯菌灵在20 x 10（-6）M或更高浓度下具有毒性。这些结果证明了斑马鱼胚胎作为体内系统来检查未知化合物的雌激素和发育毒性潜力的有用性。

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