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首页> 外文期刊>Journal of applied toxicology >Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures
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Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures

机译：微囊藻毒素-LR对不同温度下斑马鱼（Danio rerio）的毒性作用研究

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摘要

It is well known that fish have stronger tolerance than mammals to microcystin (MC) exposure, and such a difference is attributed to their different core body temperatures. However, no in vivo study has been conducted to investigate the effects of temperature on MC-induced toxicity in fish, a typical poikilotherm. Tolerance and detoxification response of zebrafish treated with MC-LR were investigated under three temperatures. The LD_(50) values evidently increased with a decline of the temperature (547,260 and 176 u,g kg1 at 12,22 and 32 degC, respectively), indicating stronger tolerance of the fish at lower temperatures. Changes in the transcription of glutathione S-transferase (GST) isoforms in the fish were observed, and their sensitivity of response in the transcription of GST mRNA was on the order of 12 > 32 > 22degC. We screened out several GST genes which were more delicate to solve the MC-LR exposure at different temperatures, i.e. GST rhoi, al, p1 and thetai in the 12 degC group, and GST zetai and p2 in the 22 and 32 degC groups. Our findings partly validate the hypothesis that high temperature enhances toxic effects of MCs on poikilotherms. Our studies also indicate that temperature-dependent toxic effects should be taken into account for field toxic assessment of microcystins in fish.
机译：众所周知，鱼类对微囊藻毒素（MC）的耐受性比哺乳动物强，而这种差异归因于它们不同的核心体温。但是，还没有进行体内研究来研究温度对MC引起的鱼类毒性的影响，这是典型的poikilotherm。在三个温度下研究了用MC-LR处理的斑马鱼的耐受性和解毒反应。 LD_（50）值明显随温度的下降而增加（分别在12,22和32℃时为547,260和176 u，g kg1），表明鱼在较低温度下的耐受性更强。观察到鱼中谷胱甘肽S-转移酶（GST）同工型的转录变化，其对GST mRNA转录的响应敏感性为12> 32> 22degC。我们筛选出了一些GST基因，它们更能解决在不同温度下的MC-LR暴露问题，例如12 degC组中的GST rhoi，al，p1和thetai，以及22和32 degC组中的GST zetai和p2。我们的发现部分验证了高温会增强MC对poikilotherms的毒性作用这一假说。我们的研究还表明，对鱼类中微囊藻毒素的田间毒性评估应考虑温度依赖性的毒性作用。

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