...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of applied toxicology >The extract of the jellyfish Phyllorhiza punctata promotes neurotoxic effects.
【24h】

The extract of the jellyfish Phyllorhiza punctata promotes neurotoxic effects.

机译：海PhPhyllorhiza punctata的提取物具有神经毒性作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phyllorhiza punctata (P. punctata) is a jellyfish native to the southwestern Pacific. Herewith we present the biochemical and pharmacological characterization of an extract of the tentacles of P. punctata. The tentacles were subjected to three freeze-thaw cycles, homogenized, ultrafiltered, precipitated, centrifuged and lyophilized to obtain a crude extract (PHY-N). Paralytic shellfish poisoning compounds such as saxitoxin, gonyautoxin-4, tetrodotoxin and brevetoxin-2, as well as several secretory phospholipase A(2) were identified. PHY-N was tested on autonomic and somatic neuromuscular preparations. In mouse vas deferens, PHY-N induced phasic contractions that reached a peak of 234 +/- 34.7% of control twitch height, which were blocked with either 100 mu m of phentolamine or 1 m m of lidocaine. In mouse corpora cavernosa, PHY-N evoked a relaxation response, which was blocked with either L-N(G) -Nitroarginine methyl ester (0.5 m m) or 1 m m of lidocaine. PHY-N (1, 3 and 10 mug ml(-1) ) induced an increase in tonus of the biventer-cervicis neuromuscular preparation that was blocked with pre-treatment of galamine (10 mu m). Administration of 6 mg kg(-1) PHY-N intramuscularly produced death in broilers by spastic paralysis. In conclusion, PHY-N induces nerve depolarization and nonspecifically increases neurotransmitter release. Copyright (c) 2011 John Wiley & Sons, Ltd.
机译：Phyllorhiza punctata（P. punctata）是一种水母，原产于西南太平洋。因此，我们提出了P. punctata触角提取物的生化和药理学表征。使触角经受三个冻融循环，均质，超滤，沉淀，离心和冻干，以获得粗提取物（PHY-N）。麻痹性贝类中毒化合物，如毒素，淋菌毒素4，河豚毒素和短毒素2，以及几种分泌型磷脂酶A（2）。 PHY-N已在自主神经和体神经肌肉制剂上进行了测试。在小鼠输精管中，PHY-N诱导的相变收缩达到对照抽搐高度的234 +/- 34.7％的峰值，并被100μm的苯妥拉明或1 mm的利多卡因阻滞。在小鼠海绵体中，PHY-N引起松弛反应，该反应被L-N（G）-硝基精氨酸甲酯（0.5 m m）或1 m m的利多卡因所阻断。 PHY-N（1、3和10毫升ml（-1））导致biventer-cervicis神经肌肉制剂的紧张度增加，而该制剂被加拉敏（10μm）预处理所阻断。在肉鸡中通过痉挛性麻痹管理6 mg kg（-1）PHY-N肌内产生死亡。总之，PHY-N诱导神经去极化，并非特异性地增加神经递质的释放。版权所有（c）2011 John Wiley＆Sons，Ltd.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号