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首页> 外文期刊>Journal of applied toxicology >The effect of methylmercury exposure on early central nervous system development in the zebrafish (Danio rerio) embryo
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The effect of methylmercury exposure on early central nervous system development in the zebrafish (Danio rerio) embryo

机译：甲基汞暴露对斑马鱼（Danio rerio）胚胎早期中枢神经系统发育的影响

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摘要

Much attention is focused on environmental contamination by heavy metals. The heavy metal mercury is found worldwide and is ranked number 3 on the Comprehensive Environmental Response, Compensation and Liability Act substance list. We examined the effect of low-level methylmercury exposure on central nervous system development of wild-type zebrafish embryos (ZFEs) of the AB strain because methylmercury is the most common form of mercury to which humans are exposed in the environment. ZFEs were exposed to nine different concentrations of methylmercury [0 (negative control), 5, 10, 50, 80, 100, 200, 500 and 1000 parts per billion (μg l -1)] starting at 6h post-fertilization, which is the time the neural tube is first beginning to form. ZFEs were exposed to 2% ethanol as positive controls (100% embryonic death). ZFEs were assessed at 30, 54, 72 and 96h post-fertilization for changes in embryonic development, mortality, time of hatching and morphological deformities. No abnormalities were observed in ZFEs exposed to 5μg l -1 methylmercury. The time of hatching from the chorion was delayed in ZFEs exposed to methylmercury concentrations of 50μg l -1 or higher. Significantly more ZFEs exposed to 0, 5 or 10μg l -1 methylmercury successfully completed hatching compared with ZFEs exposed to 50μg l -1 or higher methylmercury. ZFEs exposed to more than 200μg l -1 methylmercury exhibited 100% embryonic mortality. The rate of cell proliferation within the neural tube was significantly decreased in embryos exposed to 10, 50 and 80μg l -1 methylmercury and there were no differences between these doses.
机译：许多注意力集中在重金属对环境的污染上。重金属汞在全球范围内发现，在《综合环境响应，补偿和责任法》物质清单中排名第三。我们检查了低水平甲基汞暴露对AB株野生型斑马鱼胚胎（ZFE）的中枢神经系统发育的影响，因为甲基汞是人类在环境中接触的最常见的汞形式。从受精后6小时开始，ZFEs暴露于9种不同浓度的甲基汞[0（阴性对照），5、10、50、80、100、200、500和1000十亿分之十（μgl -1）]，即神经管第一次开始形成的时间。 ZFEs暴露于2％乙醇作为阳性对照（100％胚胎死亡）。在受精后30、54、72和96h评估ZFE，以了解胚胎发育，死亡率，孵化时间和形态畸形的变化。在暴露于5μgl -1甲基汞的ZFE中未观察到异常。暴露于甲基汞浓度为50μgl -1或更高的ZFE中，绒毛膜的孵化时间被延迟。与暴露于50μgl -1或更高甲基汞的ZFE相比，暴露于0、5或10μgl -1甲基汞的ZFE成功完成孵化的数量更多。暴露于200μgl -1甲基汞以上的ZFEs表现出100％的胚胎死亡率。在暴露于10、50和80μgl -1甲基汞的胚胎中，神经管内的细胞增殖速率显着降低，并且在这些剂量之间没有差异。

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