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首页> 外文期刊>Journal of applied toxicology >Cytotoxicity and ROS production of manufactured silver nanoparticles of different sizes in hepatoma and leukemia cells
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Cytotoxicity and ROS production of manufactured silver nanoparticles of different sizes in hepatoma and leukemia cells

机译：制备的大小不同的银纳米颗粒在肝癌和白血病细胞中的细胞毒性和活性氧的产生

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摘要

Silver nanoparticles (AgNPs), which have well-known antimicrobial properties, are extensively used in various medical and general applications. In spite of the widespread use of AgNPs, relatively few studies have been undertaken to determine the cytotoxic effects of AgNPs. The aim of this study was investigate how AgNPs of different sizes (4.7 and 42nm) interact with two different tumoral human cell lines (hepatoma [HepG2] and leukemia [HL-60]). In addition, glutathione depletion, inhibition of superoxide dismutase (SOD) and reactive oxygen species (ROS) generation were used to evaluate feasible mechanisms by which AgNPs exerted its toxicity. AgNPs of 4.7nm and 42nm exhibited a dramatic difference in cytotoxicity. Small AgNPs were much more cytotoxic than large AgNPs. A difference in the cellular response to AgNPs was found. HepG2 cells showed a higher sensitivity to the AgNPs than HL-60. However, the cytotoxicity induced by AgNPs was efficiently prevented by NAC treatment, which suggests that oxidative stress is primarily responsible for the cytotoxicity of AgNPs. Furthermore, cellular antioxidant status was disturbed: AgNPs exposure caused ROS production, glutathione depletion and slight, but not statistically significant inactivation of SOD.
机译：具有众所周知的抗菌性能的银纳米颗粒（AgNPs）被广泛用于各种医学和一般应用中。尽管AgNPs的广泛使用，但进行相对较少的研究来确定AgNPs的细胞毒性作用。这项研究的目的是研究不同大小（4.7和42nm）的AgNP如何与两种不同的肿瘤人类细胞系（肝癌[HepG2]和白血病[HL-60]）相互作用。此外，谷胱甘肽耗竭，抑制超氧化物歧化酶（SOD）和活性氧（ROS）的生成被用来评估可行的机制，AgNPs发挥其毒性。 4.7nm和42nm的AgNPs在细胞毒性方面表现出巨大的差异。小型AgNP比大型AgNP具有更大的细胞毒性。发现了细胞对AgNP的反应的差异。 HepG2细胞对AgNPs的敏感性高于HL-60。然而，NAC处理有效地预防了由AgNPs引起的细胞毒性，这表明氧化应激是造成AgNPs细胞毒性的主要原因。此外，细胞抗氧化剂的状态受到干扰：暴露于AgNPs会引起ROS的产生，谷胱甘肽耗竭以及SOD的轻微失活，但在统计学上却不显着。

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