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首页> 外文期刊>Journal of applied toxicology >Evaluation and refinement of a field-portable drinking water toxicity sensor utilizing electric cell-substrate impedance sensing and a fluidic biochip
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Evaluation and refinement of a field-portable drinking water toxicity sensor utilizing electric cell-substrate impedance sensing and a fluidic biochip

机译：利用电细胞-基质阻抗传感和流体生物芯片评估和改进便携式饮用水毒性传感器

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The US Army's need for a reliable and field-portable drinking water toxicity sensor was the catalyst for the development and evaluation of an electric cell-substrate impedance sensing (ECIS) device. Water testing technologies currently available to soldiers in the field are analyte-specific and have limited capabilities to detect broad-based water toxicity. The ECIS sensor described here uses rainbow trout gill epithelial cells seeded on fluidic biochips to measure changes in impedance for the detection of possible chemical contamination of drinking water supplies. Chemicals selected for testing were chosen as representatives of a broad spectrum of toxic industrial compounds. Results of a US Environmental Protection Agency (USEPA)-sponsored evaluation of the field portable device were similar to previously published US Army testing results of a laboratory-based version of the same technology. Twelve of the 18 chemicals tested following USEPA Technology Testing and Evaluation Program procedures were detected by the ECIS sensor within 1 h at USEPA-derived human lethal concentrations. To simplify field-testing methods further, elimination of a procedural step that acclimated cells to serum-free media streamlined the test process with only a slight loss of chemical sensitivity. For field use, the ECIS sensor will be used in conjunction with an enzyme-based sensor that is responsive to carbamate and organophosphorus pesticides. Copyright (c) 2014 John Wiley & Sons, Ltd.
机译：美国陆军对可靠且可现场携带的饮用水毒性传感器的需求，是开发和评估单电池-基板阻抗感测（ECIS）设备的催化剂。目前，该领域的士兵可使用的水测试技术是特定于分析物的，并且检测基础广泛的水毒性的能力有限。此处描述的ECIS传感器使用接种在流体生物芯片上的虹鳟tr腮上皮细胞来测量阻抗变化，以检测饮用水源可能的化学污染。选择用于测试的化学药品作为多种有毒工业化合物的代表。美国环境保护署（USEPA）赞助的便携式现场设备评估结果与先前发布的美国陆军基于实验室技术的同一技术的测试结果相似。在USEPA衍生的人类致死浓度下，由ECIS传感器在1小时内检测到了按照USEPA技术测试和评估计划程序测试的18种化学药品中的12种。为了进一步简化现场测试方法，省去了使细胞适应无血清培养基的程序步骤，从而简化了测试过程，而化学敏感性仅略有下降。对于野外使用，ECIS传感器将与对氨基甲酸酯和有机磷农药有反应的基于酶的传感器结合使用。版权所有（c）2014 John Wiley＆Sons，Ltd.

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