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首页> 外文期刊>Journal of applied toxicology >Interactive and delayed effects of pyridostigmine and physical stress on biochemical and histological changes in peripheral tissues of mice.
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Interactive and delayed effects of pyridostigmine and physical stress on biochemical and histological changes in peripheral tissues of mice.

机译：吡啶斯的明和物理应激对小鼠外周组织生化和组织学变化的相互作用和延迟作用。

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Gulf War veterans were taking pyridostigmine orally against possible exposure to nerve agents as well as being under physical stress. This study was designed to investigate the delayed effects of pyridostigmine and treadmill exercise on cholinesterase activity, lipid peroxidation and histology of peripheral tissues of mice. Male NIH Swiss mice were divided into four groups of 15 animals each and treated as follows: sedentary control; exercise training for 10 weeks; pyridostigmine (1.2 mg kg(-1), p.o.) for 2 weeks during weeks 5 and 6; and pyridostigmine plus exercise training. The mice were sacrificed 24 h after the last exercise, and blood, triceps muscle and sciatic nerve were isolated and analyzed. The group treated with pyridostigmine alone showed decreased plasma butyrylcholinesterase (BChE) activity (87% of control), whereas pyridostigmine plus exercise significantly decreased the BChE activity (79% of control), indicating an interactive effect of the combination. Acetylcholinesterase (AChE) activity did not alter significantly in red blood cells, platelets or sciatic nerve with either of the treatments. However, AChE activity in triceps muscle decreased significantly (78% of control) in the group treated with pyridostigmine plus exercise. Creatine phosphokinase activity in plasma increased slightly (compared to control, pyridostigmine or exercise group) in mice treated with pyridostigmine plus exercise, which may be indicative of perturbation in the integrity of the skeletal muscle due to combination. However, there were no obvious histological abnormalities in the triceps muscle detected between experimental and control groups. Interaction of pyridostigmine and exercise significantly increased the concentration of the end product of lipid peroxidation (malondialdehyde) (124% of control) in triceps muscle, indicating an oxidative stress response of the combination. These results indicate that physical stress enhanced the delayed toxic effects of a subchronic oral dose of pyridostigmine primarily in the skeletal muscle of mice. Copyright 2000 John Wiley & Sons, Ltd.
机译：海湾战争退伍军人正在口服吡啶斯的明，以防可能接触神经毒剂以及处于身体压力下。本研究旨在研究吡啶斯的明和跑步机运动对小鼠胆碱酯酶活性，脂质过氧化作用和周围组织组织学的延迟影响。将雄性NIH Swiss小鼠分成四组，每组15只动物，并按以下方式处理：久坐对照;和运动训练10周;在第5周和第6周持续2周使用吡啶斯的明（1.2 mg kg（-1），p.o.）;和吡ido斯的明加上运动训练。在最后一次运动后24小时处死小鼠，并分离和分析血液，三头肌和坐骨神经。单独用吡啶斯的明治疗的组显示血浆丁酰胆碱酯酶（BChE）活性降低（对照的87％），而吡啶斯的明加运动显着降低了BChE活性（对照的79％），表明该组合具有交互作用。两种治疗方法之一，红细胞，血小板或坐骨神经中的乙酰胆碱酯酶（AChE）活性均无明显变化。但是，在使用吡ido斯的明加运动治疗的组中，三头肌肌肉中的AChE活性显着下降（占对照组的78％）。在用吡tig斯的明加运动治疗的小鼠中血浆中的肌酸磷酸激酶活性略有增加（与对照组，吡mine斯的明或运动组相比），这可能表明骨骼肌的完整性受到了干扰。然而，实验组和对照组之间未检测到三头肌的明显组织学异常。吡啶斯的明的相互作用和运动显着增加了肱三头肌中脂质过氧化终产物（丙二醛）的浓度（对照组的124％），表明该组合具有氧化应激反应。这些结果表明，物理应激增强了次慢性口服剂量的吡啶斯的明的延迟毒性作用，主要在小鼠的骨骼肌中。版权所有2000 John Wiley＆Sons，Ltd.

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