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首页> 外文期刊>Journal of applied toxicology >Gene expression analyses of vitellogenin, choriogenin and estrogen receptor subtypes in the livers of male medaka (Oryzias latipes) exposed to equine estrogens
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Gene expression analyses of vitellogenin, choriogenin and estrogen receptor subtypes in the livers of male medaka (Oryzias latipes) exposed to equine estrogens

机译：暴露于马雌激素的男性花aka（Oryzias latipes）肝脏中卵黄蛋白原，绒毛膜上皮激素和雌激素受体亚型的基因表达分析

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In the present study, we investigated transcriptional profiles of estrogen-responsive genes, such as vitellogenins (Vtg1 and Vtg2), choriogenins (ChgL and ChgH) and estrogen receptor subtypes (ER, ER1, and ER2), in the liver of male medaka fish (Oryzias latipes) that were exposed to six equine estrogens (1-300 ng l(-1)) for 3 days. Our quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses revealed that the expression levels of hepatic Vtg, Chg and ER genes in male medaka responded to various types and concentrations of equine estrogens. The estrogenic potentials of the tested chemicals were in the order of equilin > 17-estradiol > equilenin > 17-dihydroequilin > 17-dihydroequilenin > 17-dihydroequilin > 17-dihydroequilenin, showing the higher estrogenic potential of equilin than that of 17-estradiol. Our results also showed that the estrogenicities of 17-dihydroequilin and 17-dihydroequilenin were more potent than that of 17-dihydroequilin and 17-dihydroequilenin. Furthermore, in gene expression analyses of hepatic ER subtypes, observations were made to note that 17-estradiol and equilin induced ER transcription in male medaka, and the ER transcription level had significantly positive correlations with the expression of Vtg and Chg genes. In contrast, in the same 17-estradiol and equilin treatment groups, it was shown that the transcription levels of hepatic ER1 and/or ER2 had significantly negative correlations with the expression of Vtg and Chg genes. These results suggested some potential involvement of the ER subtypes in the regulation of Vtg and Chg gene expressions in the liver. This is the first report describing the comprehensive analyses of in vivo estrogenicity of the equine estrogens in male medaka. Copyright (c) 2016 John Wiley & Sons, Ltd.
机译：在本研究中，我们调查了雄性aka鱼肝脏中雌激素反应性基因的转录谱，例如卵黄蛋白原（Vtg1和Vtg2），卵母蛋白（ChgL和ChgH）和雌激素受体亚型（ER，ER1和ER2）。 （Oryzias latipes）暴露于六个马雌激素（1-300 ng l（-1））3天。我们的定量逆转录聚合酶链反应（RT-PCR）分析表明，男性中肝脏Vtg，Chg和ER基因的表达水平对马雌激素的各种类型和浓度有反应。被测化学物质的雌激素潜力依次为马匹> 17-雌二醇>马匹宁> 17-二氢马匹宁> 17-二氢马匹宁> 17-二氢马匹宁> 17-二氢马匹宁，显示马匹的雌激素潜能高于17-雌二醇。我们的研究结果还表明，17-二氢安神宁和17-二氢安神宁的雌激素性比17-二氢安神宁和17-二氢安神宁的雌激素作用更强。此外，在肝ER亚型的基因表达分析中，观察到注意到17-雌二醇和马尿素诱导雄性花中的ER转录，并且ER转录水平与Vtg和Chg基因的表达显着正相关。相反，在相同的17-雌二醇和马尿素治疗组中，显示肝ER1和/或ER2的转录水平与Vtg和Chg基因的表达显着负相关。这些结果表明，ER亚型可能参与了肝脏中Vtg和Chg基因表达的调节。这是第一份描述雄性马六甲马雌激素体内雌激素水平的综合报告。版权所有（c）2016 John Wiley＆Sons，Ltd.

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